Background: Single domain antibodies from camel heavy chain antibodies (VHH or nanobody), are advantages due to higher solubility, stability, high homology with human antibody, lower immunogenicity and low molecular weight. These criteria make them candidates for production of engineered antibody fragments particularly in transgenic animals. Objective: To study the development of transgenic ch...

We have described a mouse T cell alloantigen, Tsud, 1 which may be an allotypic or idiotypic determinant on a T cell receptor (1-3). Tsu d is associated with the T cell receptor for idiotype, apparent ly on suppressor T cells or their precursors (2). The determinant is defined by antisera made in B A L B / c mice against T cell blasts from allotype congenic C.AL-20 mice and can be detected by i...

background: evaluation on the toxicity of two mainly contaminant heavy metals, cadmium (cd) and lead (pb) through the food chain was the aim of this study. methods: a total number of 270 healthy common carp (4±1.14 g) in april, 2015 transported to the khatam alanbia university of technology, behbahan, iran. fishes were divided into three groups and transferred to the 20 l aquaria each containin...

Immunoproliferative small intestinal disease (IPSID) was recently added to the growing list of infectious pathogen-associated human lymphomas. Molecular and immunohistochemical studies demonstrated an association with Campylobacter jejuni. IPSID is a variant of the B-cell lymphoma of mucosa-associated lymphoid tissue (MALT), which involves mainly the proximal small intestine resulting in malabs...

Evidence derived from the complete amino acid sequences of the variable regions of both the heavy and light chains of two members (BOR and KAS) of the Wa idiotypic family of human rheumatoid factors suggests that not only are the light chains of these molecules derived from possibly one variable region gene segment, but the heavy chain variable regions are all derived from the VHI subgroup of h...

Formation of a complete immunoglobulin heavy-chain transcription unit involves the ordered rearrangement of variable (V), diversity (D), and joining (J) region gene segments. In antibody-producing cells, this process is regulated such that only one of two antibody genes is expressed. Experiments with transgenic mice suggest that this mechanism, known as allelic exclusion, is mediated through th...

Immunoproliferative small intestinal disease (IPSID) was recently added to the growing list of infectious pathogen-associated human lymphomas. Molecular and immunohistochemical studies demonstrated an association with Campylobacter jejuni. IPSID is a variant of the B-cell lymphoma of mucosa-associated lymphoid tissue (MALT), which involves mainly the proximal small intestine resulting in malabs...

The carbohydrate moiety of HLA antigens was radioactively labeled in galactose residues by treatment of membranes with neuraminidase, galactose oxidase, and NaB”H,. A single N-linked oligosaccharide side chain M, 2700 to 3300 was labeled by this treatment. Unique pronase and tryptic “H-glycopeptides were purified, using lentil-lectin affinity chromatography. They contained all the radioactivity...

There is strong interest in the design of bispecific monoclonal antibodies (bsAbs) that can simultaneously bind 2 distinct targets or epitopes to achieve novel mechanisms of action and efficacy. Multiple bispecific formats have been proposed and are currently under development. Regeneron's bispecific technology is based upon a standard fully human IgG antibody in order to minimize immunogenicit...