BACKGROUND Dengue fever results from infection with one or more of four different serotypes of dengue virus (DENV). Despite the widespread nature of this infection, available molecular diagnostics have significant limitations. The aim of this study was to develop a multiplex, real-time, reverse transcriptase-PCR (rRT-PCR) for the detection, quantitation, and serotyping of dengue viruses in a si...

osteosarcoma is a relatively uncommon malignancy however, it is the most common form of primary malignant bone tumors in human. diagnosis and prognosis of patients with osteosarcoma is limited to clinico-radiopathological parameters, whereas molecular markers of tumor aggression have been poorly identified. survivin, an inhibitor of apoptosis (iap), is unique for its expression in human tumors ...

abstract background and aim: survivin is a new member of inhibitor of apoptosis protein family (iap) that plays an important role in the regulation of cell cycle and inhibition of apoptosis. distinct expression of this gene in tumoral cells versus normal cells introduces it as the fourth major transcriptome in cancers. thyroid carcinoma is the most common endocrine malignancy. considering the h...

background: respiratory virus infections in children are a leading cause of morbidity and mortality worldwide. methods : a total of 897 clinical specimens were collected from february 2007 to january 2008 and transported to the na­tional influenza center. two hundred and two samples belonged to children under the age of six from 897 specimens, de­scribed above, were selected. then they were tes...

BACKGROUND Respiratory virus infections in children are a leading cause of morbidity and mortality worldwide. METHODS A total of 897 clinical specimens were collected from February 2007 to January 2008 and transported to the National Influenza Center. Two hundred and two samples belonged to children under the age of six from 897 specimens, described above, were selected. Then they were tested...

Detection of African horsesickness virus (AHSV) by three different reverse transcription polymerase chain reaction (RT-PCR) based methods were compared. Primers were complementary to sequences contained in the gene encoding non-structural protein 2 of AHSV serotype 3. All three assays were group-specific for AHSV and detected all nine serotypes but not related orbiviruses. A dilution range was ...

Three multiplex hemi-nested RT-PCR assays were developed to detect simultaneously 12 RNA respiratory viruses: influenza viruses A, B and C, human respiratory syncytial virus (hRSV), human metapneumovirus (hMPV), parainfluenza virus types 1-4 (PIV-1, -2, -3 and -4), human coronavirus OC43 and 229E (HCoV) and rhinovirus (hRV). An internal amplification control was included in one of the RT-PCR as...

A new method for specific reamplification of DDRT-PCR products is presented. After transient ligation of the primary DDRT-PCR fragments into a T-vector, the cDNAs of interest were reamplified by hemi-nested PCR and thermally asymmetric cycles. In contrast to the originally described protocol, this method of reamplification is specific, sensitive, reproducibly gives a high yield of DNA and allow...