MicroRNAs has been shown to regulate lipogenesis in liver. The aim of the present study was to investigate whether the effects of resveratrol (RSV) on lipogenesis is associated with the changes in the expression of two miRNAs (miR-107 and miR-10b) that regulate lipogenic pathways. 30 wild type C57BL/6j male mice were randomly fed three diets: a standard chow diet (ND), a high fat diet (HFD, 60%...

a new series of 1,2-diaryl-4,5,6,7-tetrahydro-1h-benzo[d]imidazoles, possessing trimethoxy phenyl pharmacophore, were synthesized to evaluate their biological activities as tubulin inhibitors. cytotoxic activity of the synthesized compounds 7a-f was assessed against several human cancer cell lines, including mcf-7 (breast cancer cell), hepg2 (liver hepatocellular cells), a549 (adenocarcinomic h...

OBJECTIVE To explore the potential role of CpG motif-containing oligonucleotides (CpG-ODN) in modulating the expression of FasL in HepG2 and Fas in Jurkat cells in vitro, and to examine the effect of CpG-ODN treatment on the HepG2 cells-mediated Jurkat cell apoptosis in vitro. METHODS The expressions of FasL in HepG2 and Fas in Jurkat cells were examined by real time PCR and flow cytometry (F...

We report the spatiotemporal control of a 3D coculture by combining photocurable and chemically degradable hydrogels for coculture of embryonic stem (ES) cells with HepG2 cells. ES cells were encapsulated in micropatterned photocurable poly(ethylene glycol) (PEG) hydrogels. The cell-laden PEG hydrogels were then encapsulated in calcium-alginate (Ca-Alg) hydrogel containing HepG2 cells. The ES c...

AIMS The aim was develop stable human cell line stable over-expressing transcription co-activator peroxisome proliferator-activated receptor gamma co-activator 1α (PGC-1α) with restored hepatospecific functions and increased expression of major xenobiotic metabolizing enzymes. METHODS Six clones of HepG2-PGC-1α and one control clone HepG2-pcDNA3 were isolated and analyzed for secretion of hep...

This study was conducted to evaluate the effect of mesenchymal stem cells (MSCs) and a novel curcumin derivative (NCD) on HepG2 cells (hepatoma cell line) and to investigate their effect on Notch1 signaling pathway target genes. HepG2 cells were divided into HepG2 control group, HepG2 cells treated with MSC conditioned medium (MSCs CM), HepG2 cells treated with a NCD, HepG2 cells treated with M...

Acesulfame potassium (ACE-K) is an artificial sweetener widely used in many foods. This investigation assessed the cytotoxic effect of ACE-K using MTT assay human hepatocellular carcinoma (HepG2) cell line and genotoxic chromosomal aberrations (CAs), micronucleus (MN), comet assays lymphocytes. 7.5-240 ?g/mL concentrations were applied to cells. notably decreased viability on HepG2 cells, espec...