We have followed the in vitro degradation of rat histidine decarboxylase in a reconstituted system, containing only rat histidine decarboxylase (obtained by in vitro transcription and translation), calcium ions in the millimolar range of concentrations, and m-calpain. Under the experimental conditions used, m-calpain quickly and efficiently degraded rat histidine decarboxylase, giving rise to a...

A method of determining histidine decarboxylase activity was established. In this method, 14C-histamine was separated from 14C-histidine by Amberlite CG-resin column in the ammonium form. Lecanoric acid was obtained by screening histidinedecarboxylase inhibitors produced by microorganisms. It is the first isolation of this compound from fungi. The inhibition by lecanoric acid was competitive wi...

Histidine decarboxylase from fetal rat liver was purified to near-homogeneity. The purified enzyme has a molecular weight of 210,000, and appears to contain two subunits with molecular weights of 145,000 and 66,000, respectively. The enzyme is inhibited by heavy metals such as Hg2+ and Zn2+ and sulfhydryl-reactive compounds such as 5,5'-dithiobis-2-nitrobenzoic acid. The enzyme is partially dep...

For a long time the structural and molecular features of mammalian histidine decarboxylase (EC 4.1.1.22), the enzyme that produces histamine, have evaded characterization. We overcome the experimental problems for the study of this enzyme by using a computer-based modelling and simulation approach, and have now the conditions to use histidine decarboxylase as a target in histamine pharmacology....

Hydrocortisone-stimulated synthesis of histidine decarboxylase (EC 4.1.1.22) was studied in rat stomach. Single or daily administration of hydrocortisone increased the histidine decarboxylase activity but not the histamine content in the fundic glands of stomachs from fed and fasted rats with or without adrenalectomy, and also in those from adrenalectomized fasted rats with concomitant treatmen...

Molecular modeling analysis of charge density and heat of fornation by PM3 method as well as C, H NMR and 2D-NMR measurements of histidine (substrate) and some of its derivatives as histidine decarboxylase inhibitors were performed. It was established that the atom, usually nitrogen, which forms internal aldimine with pyridoxal5 -phosphate (PLP), (coenzyme), has negative and almost equal ...

molecular modeling analysis of charge density and heat of fornation by pm3 method as well as c, h nmr and 2d-nmr measurements of histidine (substrate) and some of its derivatives as histidine decarboxylase inhibitors were performed. it was established that the atom, usually nitrogen, which forms internal aldimine with pyridoxal5 -phosphate (plp), (coenzyme), has negative and almost equal charge...

Histamine intoxication is an important food safety and public health concern. Ripened cheeses are the most common dairy products in which histamine can accumulate. formed by microbiota present cheese decarboxylation of histidine, due to action histidine decarboxylase. This study's objective was identify responsible for formation accumulated commercial cheeses. The content 39 different types mar...