Here, we report complete genome sequences of grapevine yellow speckle viroid 1 (GYSVd1) and hop stunt viroid (HSVd), members of the family Pospiviroidae, assembled from the transcriptome data generated from Ixeridium dentatum plants. To our knowledge, this is the first report of GYSVd1 and HSVd in I. dentatum.

Hop stunt viroid (HSVd), a significant pathogen of some hop cultivars, was detected for the first time in hops Australia. Plants were tested graft-transmissible pathogens prior to their use research trials. Upon observing symptoms indicative potential infection, RNA extracted and amplified using reverse transcription polymerase chain reaction (RT-PCR); HSVd identity confirmed by sequencing.

Sweet lime split bark is a disorder of increasing importance in Fars province. Previously we showed that a new variant of hop stunt viroid (HSVd-sb) was related to the disorder. It had homology with a noncachexia variant of hop stunt viroid (CVd-IIa-117) which causes cracking the bark of trifoliate orange rootstocks. In this study, showed that M.NgoBIX, BglI and BsrI restriction enzymes can c...

Abstract Background and Aims: Hop stunt viroid (HSVd, genus Hostuviroid, family Pospiviroidae) has a wide host range among trees and herbaceous plants. The objectives of this study were to compare biological and physical characteristics of three isolates of HSVd from mulberry, fig and citrus and search for other hosts of this viroid in Iran. Materials and Methods: Plant samples were col...

sweet lime split bark is a disorder of increasing importance in fars province. previously we showed that a new variant of hop stunt viroid (hsvd-sb) was related to the disorder. it had homology with a noncachexia variant of hop stunt viroid (cvd-iia-117) which causes cracking the bark of trifoliate orange rootstocks. in this study, showed that m.ngobix, bgli and bsri restriction enzymes can cut...

abstract hop stunt viroid (hsvd) and citrus tristeza virus (ctv) are the causal agents of most important diseases of citrus. previously we reported a novel variant of hsvd that constantly associated with the yellow corky vein disease. here, 19 individual citrus leaves with yellow corky vein symptoms were sampled for ctv by elisa. in this study, most symptomatic samples were found to be infected...

Fifteen years after transfer to hops, hop stunt viroid-grapevine (HSVd-g) was replaced by HSVd-hop (HSVd-h), a sequence variant that contains changes at five different positions. HSVd-g54 is a laboratory mutant derived from HSVd-g that differs from its progenitor by a single G to A substitution at position 54. While infection by HSVd-h induces only mild stunting in cucumber (Cucumis sativus L.)...

Weed plants characteristic for potato and hop fields have not been considered in the past as potential hosts that could transmit and lead to spreading of potato spindle tuber (PSTVd) and hop stunt (HSVd) viroids, respectively. To gain insight into this problem, we biolistically inoculated these weed plants with viroid populations either as RNA or as cDNA. New potential viroid host species, coll...

Hop stunt viroid (HSVd) is able to infect a number of herbaceous and woody hosts, such as grapevine, Citrus or Prunus plants. Previous phylogenetic analyses have suggested the existence of three major groups of HSVd isolates (plum-type, hop-type and citrus-type). The fact that these groups often contain isolates from only a limited number of isolation hosts prompted the suggestion that group-di...

The Sudanese citrus industry is mainly based on oldline cultivars of grapefruit, sweet orange and willow leaf mandarin, grafted almost exclusively on sour orange. Infection with graft-transmissible agents, viroids in particular, may have deleterious effects on the productivity of these citrus species and, more importantly, limits the choice of rootstocks should sour orange be replaced by altern...