Background and Aims: Hemophilia is a rare autoimmune disorder caused by autoantibodies directed in the majority of the cases against clotting factor VIII (FVIII). FVIII is extracted from human plasma or engineered from mammalian cell cultures using recombinant DNA technology. In Iran, most of the used FVIII is prepared from human plasma in Iranian Blood Transfusion Organization. It was seen imp...

thrombotic thrombocytopenic purpura is the result of multiple thrombus disseminated in small arterioles throughout the body. principle damage is in the endothelium of small vessels and capillaries. from 1982 to 1999, 28 patients (16 female and 12 male) have been treated for ttp. the outcome was evaluated for risk factors and prognosis. these patients were 18 to 69 years old. all had neurologica...

A simple method for isolation of kallikrein from human plasma is described. Before activation of the enzyme with acetone, the plasma was treated with 0.2 M-methylamine at pH 8.2 to inactivate a2-macroglobulin and thus prevent the irreversible binding of the active enzyme to the inhibitor. The enzyme was adsorbed on soya-bean trypsin inhibitor-Sepharose 4B and eluted with 5 mM-NaOH, pH 11.3. It ...