The routine quality control of a herbal drug preparation requires the identification and determination of the contents of the active ingredients in the product. Measurement alone is not sufficient; it is also necessary to know how objectively to evaluate the experimental results . There are 6 Hyoscyamus (Solanaceae) species growing in Turkey, none of which are endemic. Hyoscyamus (Solanaceae) s...

The purpose of the present study was to determine the tropane alkaloid content of four Hyoscyamus species from Iran, i.e. H. pusillus L., H. niger L., H. reticulatus L. and H. kurdicus Bornm. Determination of alkaloids was performed by the HPLC method. Samples were extracted with chloroform-methanol-25% ammonium hydroxide 15:5:1 (V/V/V). HPLC separation was performed on Eurospher C18 reversed p...

Background and objectives: Hyoscyamus reticulatus contains two distinguished tropane alkaloids, hyoscyamineand scopolamine and both of the compounds possesspotential acute or chronic toxicity. In the present study, a simple and efficient transformation system was established for in vitro hairy roots induction in Hyoscyamus reticulatus. M...

background and objectives: hyoscyamus reticulatus contains two distinguished tropane alkaloids, hyoscyamineand scopolamine and both of the compounds possesspotential acute or chronic toxicity. in the present study, a simple and efficient transformation system was established for in vitro hairy roots induction in hyoscyamus reticulatus. methods: effect of different factors including agrobacteriu...

the purpose of the present study was to determine the tropane alkaloid content of four hyoscyamus species from iran, i.e. h. pusillus l., h. niger l., h. reticulatus l. and h. kurdicus bornm. determination of alkaloids was performed by the hplc method. samples were extracted with chloroform-methanol-25% ammonium hydroxide 15:5:1 (v/v/v). hplc separation was performed on eurospher c18 reversed p...

The purpose of the present study was to determine the tropane alkaloid content of four Hyoscyamus species from Iran, i.e. H. pusillus L., H. niger L., H. reticulatus L. and H. kurdicus Bornm. Determination of alkaloids was performed by the HPLC method. Samples were extracted with chloroform-methanol-25% ammonium hydroxide 15:5:1 (V/V/V). HPLC separation was performed on Eurospher C18 reversed p...