We have previously identified mutant alleles of genes encoding two Rab proteins, Ypt3 and Ryh1, through a genetic screen using the immunosuppressant drug FK506 in fission yeast. In the same screen, we isolated gdi1-i11, a mutant allele of the essential gdi1+ gene encoding Rab GDP-dissociation inhibitor. In gdi1-i11, a conserved Gly267 was substituted by Asp. The Gdi1G267D protein failed to extr...

Biosynthesis of locust apolipophorin-111 (apo-111) was studied in vitro. Gel electrophoresis and immunoblotting analyses of the locust hemolymph demonstrated that apo-111 first appears in the hemolymph on the day 3 of the adult stage after the final molt and its hemolymph concentration increases thereafter. When incubated in vitro in a medium containing radioactive amino acid, the fat body cell...

We present an investigation of Er photoluminescence in Y2O3 waveguides codoped with Eu . As a function of europium concentration we observe an increase in decay rate of the erbium I11/2 energy level and an increase of the ratio of photoluminescence emission from the I13/2 and I11/2 states. Using a rate equation model, we show that this is due to an energy transfer from the I11/2→I13/2 transitio...

The enzyme catalyzing this reaction has been named UDP-G1cNAc:glycopeptide 84-N-acetylglucosaminyltransferase I11 (GlcNAc-transferase III) to distinguish it from two other GlcNAc-transferases (I and 11) present in hen oviduct and previously described in several mammalian tissues. GlcNAc-transferases I and 11, respectively, attach GlcNAc in 81-2 linkage to the Manal-3 and Manal-6 arms of Asn-lin...

After the discovery of a unique pentasaccharide domain in heparin that is responsible for activation of antithrombin 111, many analogues have been synthesised which stimulate the inhibition of factor Xa. Some aspects of the synthesis of heparin like pentasaccharides are presented. Furthermore, pentasaccharide conjugates could be designed and synthesised which stimulate the antithrombin I11 medi...

Objective. To determine the origin, distribution, and biologic fate of platelet-derived connective tissue activating peptid+III (CTAP-111), to define the relative amounts of the antigen forms (CTAP-111, betathromboglobulin [@-TG], neutrophil activating peptide-2 [NAP-21) in plasma of normal persons and those with rheumatic or end-stage renal disease, and to define the isoforms of CTAP-I11 in pl...

Apolipoprotein C-I11 (apoC-111) is a major protein of very low density lipoprotein (VLDL). The apoC-I11 polypeptide contains a carbohydrate chain containing galactosamine, galactose, and sialic acid attached in 0-linkage to a threonine residue at position 74. We have cloned the apoC-I11 gene from a subject whose serum contained unusually high amounts of apoC-111 lacking the carbohydrate moiety ...

A sequential Bayesian phase II/III design is proposed for comparative clinical trials. The design is based on both survival time and discrete early events that may be related to survival and assumes a parametric mixture model. Phase I1 involves a small number of centers. Patients are randomized between treatments throughout, and sequential decisions are based on predictive probabilities of conc...