نتایج جستجو برای: ibtx

تعداد نتایج: 96  

Journal: :Journal of neurophysiology 2013
Mark D Benton Amanda H Lewis Jason S Bant Indira M Raman

Purkinje cells have specialized intrinsic ionic conductances that generate high-frequency action potentials. Disruptions of their Ca or Ca-activated K (KCa) currents correlate with altered firing patterns in vitro and impaired motor behavior in vivo. To examine the properties of somatic KCa currents, we recorded voltage-clamped KCa currents in Purkinje cell bodies isolated from postnatal day 17...

2015
BRANDON M. SCHICKLING SARAH K. ENGLAND NUKHET AYKIN-BURNS LYSE A. NORIAN KIMBERLY K. LESLIE VICTORIA P. FRIEDEN-KOROVKINA

In breast cancers, the large conductance Ca2+ and voltage sensitive K+ (BKCa) channels have been hypothesized to function as oncoproteins, yet it remains unclear how inhibition of channel activity impacts oncogenesis. We demonstrated herein that iberiotoxin (IbTX), an inhibitor of BKCa channels, differentially modulated the in vitro tumorigenic activities of hormone-independent breast cancer ce...

Journal: :Cardiovascular research 1999
L Bang S Boesgaard J E Nielsen-Kudsk N G Vejlstrup J Aldershvile

OBJECTIVE Recent in vitro data suggest, large conductance calcium-activated K+ channels (BKCa) modulate the vascular response to nitric oxide (NO). The in vivo implications and the characteristics of this interaction are not clear. This study firstly investigates whether modulation of BKCa affects the vascular response to nitroglycerin (NTG)-derived NO in vivo and in the isolated heart and seco...

Journal: :FEBS letters 2002
Nathan Schroeder Theodore J Mullmann W A Schmalhofer Ying-Duo Gao Maria L Garcia Kathleen M Giangiacomo

Iberiotoxin (IbTX) is a remarkably selective alpha-K toxin peptide (alpha-KTx) inhibitor of the maxi-K channel. In contrast, the highly homologous charybdotoxin inhibits both the maxi-K and K(V)1.3 channels with similar high affinity. The present study investigates the molecular basis for this specificity through mutagenesis of IbTX. The interactions of mutated peptides with maxi-K and K(V)1.3 ...

2017
Yi Wang Gary G Deng Kelvin P Davies

There is increasing evidence for a role of MaxiK potassium channel-activity in regulating the metabolism and intracellular signaling of non-contractile bladder mucosal tissues. At present however no studies have determined the impact of urothelial MaxiK-activity on overall bladder metabolism. To address this we have investigated the effect of bladder lumen instillation of the MaxiK inhibitor, i...

Journal: :American journal of physiology. Heart and circulatory physiology 2010
Jennifer M Hughes Melissa A Riddle Michael L Paffett Laura V Gonzalez Bosc Benjimen R Walker

The systemic vasculature exhibits attenuated vasoconstriction following hypobaric chronic hypoxia (CH) that is associated with endothelium-dependent vascular smooth muscle (VSM) cell hyperpolarization. We hypothesized that increased activity of endothelial cell (EC) large-conductance, calcium-activated potassium (BK(Ca)) channels contributes to this response. Gracilis resistance arteries from h...

Journal: :The American journal of physiology 1998
Anne Marie F Salapatek Yu-Fang Wang Yu-Kang Mao Masataka Mori Edwin E Daniel

Depolarization elicited outward K+ currents from canine lower esophageal sphincter (LES) muscle cells, primarily through iberiotoxin (IbTX)- and tetraethylammonium-sensitive Ca2+-dependent K+ channels. Current magnitudes varied with pipette Ca2+concentration (EC50 = 108.5 nM). N G-nitro-l-arginine (l-NNA, 10-4 M), IbTX (10-8 M), or buffering intracellular Ca2+ to 8 nM decreased outward currents...

2014
Angela Curci Antonietta Mele Giulia Maria Camerino Maria Maddalena Dinardo Domenico Tricarico

Here we investigated on the role of the calcium activated K(+)-channels(BKCa) on the regulation of the neuronal viability. Recordings of the K(+)-channel current were performed using patch-clamp technique in human neuroblastoma cells (SH-SY5Y) in parallel with measurements of the cell viability in the absence or presence of the BKCa channel blockers iberiotoxin(IbTX) and tetraethylammonium (TEA...

Journal: :Cardiovascular research 2011
Yangzhen Shao Reginald Liew Bingcai Guan Philip E H Wong Winston S N Shim Yeowleng Chua Guowei He

AIMS In this study, we investigated and compared the electrophysiological and molecular properties of large-conductance Ca(2+)-activated K(+) (BK(Ca)) channels between human internal mammary arteries (IMA) and radial arteries (RA). METHODS AND RESULTS IMA and RA sections were obtained from 79 patients (including 9 females) undergoing coronary artery bypass graft surgery. We examined the effec...

Journal: :American journal of physiology. Gastrointestinal and liver physiology 2012
Jin Zhang Susan T Halm Dan R Halm

Secretagogues acting at a variety of receptor types activate electrogenic K(+) secretion in guinea pig distal colon, often accompanied by Cl(-) secretion. Distinct blockers of K(Ca)1.1 (BK, Kcnma1), iberiotoxin (IbTx), and paxilline inhibited the negative short-circuit current (I(sc)) associated with K(+) secretion. Mucosal addition of IbTx inhibited epinephrine-activated I(sc) ((epi)I(sc)) and...

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