the beet moth, scrobipalpa ocellatella (boyd) (lepidoptera: gelechiidae) is one of the most destructive pests of beet. the insect overwinters as larvae of different instars in beets which are left in the field. supercooling point (scp) of individual larvae showed broad range variation from -6 to -25 °c. in the present study, factors affecting the broad range of scp were investigated. larvae sho...

It is surprisingly difficult to freeze water. Almost all ice that forms under "mild" conditions (temperatures > -40 degrees C) requires the presence of a nucleating agent--a solid particle that facilitates the freezing process--such as clay mineral dust, soot or bacteria. In a computer simulation, the presence of such ice nucleating agents does not necessarily alleviate the difficulties associa...

The simple linear polymer polyglycerol (PGL) was found to apparently bind and inhibit the ice nucleating activity of proteins from the ice nucleating bacterium Pseudomonas syringae. PGL of molecular mass 750 Da was added to a solution consisting of 1 ppm freeze-dried P. syringae 31A in water. Differential ice nucleator spectra were determined by measuring the distribution of freezing temperatur...

9,10-Dihydro-9,10-ethano-anthracene-11,12-dicarboxylic acid disodium salt (DHEAS) was synthesized and used as a nucleating agent for poly(1-butene) (iPB). The isothermal crystallization kinetics of iPB having different nucleating agents were investigated by differential scanning calorimetry (DSC) and polarized optical microscopy (POM). The results showed that the nucleating agents increased the...

The survival of freeze-tolerant vertebrates requires that ice nucleation in tissues be initiated at relatively high temperatures. Three current hypotheses for the initiation of freezing in these animals are: a) inoculation by contact with ice crystals in the external environment; b) nucleation by ice-nucleating bacteria associated with the animal; and c) nucleation by icenucleating proteins in ...

Ice nucleation and growth is an important and widespread environmental process. Accordingly, nature has developed means to either promote or inhibit ice crystal formation, for example ice-nucleating proteins in bacteria or ice-binding antifreeze proteins in polar fish. Recently, it was found that birch pollen release ice-nucleating macromolecules when suspended in water. Here we show that birch...

Regulation of the temperature at which insects freeze is a critical factor in their overwintering survival. Although the role of ice-nucleating active bacteria in promoting frost-damage in plants is well known, their interaction with other organisms has not been described. In winter. many freeze-susceptible insects avoid freezing by lowering the temperature at which their body fluids spontaneou...

A novel ice-nucleating bacterium (INB) was isolated from Ross Island, Antarctica. INBs could be isolated more frequently than was generally thought. INB strain IN-74 was found in the white colony group. Strain IN-74 was identified from its taxonomic characteristics as a novel INB, Pseudomonas antarctica IN-74. When strain IN-74 was cultured aerobically in a medium consisting of the ice-nucleati...

This manuscript presents the characterization of individual ice nucleating and ice residual particles using SEM/EDX analysis. The ice nucleating particle and ice particle residuals were collected by three different instruments. This study provides very useful information/insights on the comparison of sampling instruments/techniques for ice nuclei. The subject of the manuscript is suitable for p...

9,10-dihydro-9,10-ethano-anthracene-11,12-dicarboxylic acid disodium salt (dheas) was synthesized andused as a nucleating agent for poly(1-butene) (ipb). the isothermal crystallization kinetics of ipb havingdifferent nucleating agents were investigated by differential scanning calorimetry (dsc) and polarized opticalmicroscopy (pom). the results showed that the nucleating agents increased the cr...