نتایج جستجو برای: indirect sandwich elisa

تعداد نتایج: 132606  

Journal: :Journal of clinical microbiology 1984
B A McClane R J Strouse

Clostridium perfringens type A enterotoxin was specifically detected and readily quantified by indirect and four-layer sandwich enzyme-linked immunosorbent assays (ELISAs). With the indirect ELISA, enterotoxin was detected in quantities of as low as 2.5 ng (25 ng/ml). When the more sensitive sandwich ELISA procedures was used, 100 pg (1 ng/ml) of enterotoxin was detected. The sandwich ELISA pro...

Journal: :archives of razi institute 2016
m. torabi a. haadi e. asli m. aminian f. esmaily

in the present study, seven isolates of haemophilus influenzae type b were cultured in four different media to compare growth rate and capsule production. four liquid media namely brain heart infusion broth (bhi), trypticase soy broth (tsb), mueller hinton broth (mhb) and gonococci broth (gc) with added supplements (1% hemoglobin, 1% isovitalex) were used. the growth was measured by colony coun...

Journal: :Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 1996

Journal: :Clinical and vaccine immunology : CVI 2012
Meng Ge Wei Luo Daliang Jiang Runcheng Li Wenwei Zhao Guoliang Chen Xingdong Yang Xinglong Yu

A double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) is described for detection of porcine circovirus 2 (PCV2) antibodies using the well-characterized recombinant PCV2 capsid protein. In a comparative test of 394 pig sera against an indirect immunofluorescence (IIF) test and a commercial ELISA kit (also based on the recombinant PCV2 capsid protein), the results showed that the di...

2006
Valerie Fournier James R. Hagler Kent M. Daane Jesse H. de León Russell L. Groves Heather S. Costa Thomas J. Henneberry

The recent invasion of southern California by the glassy-winged sharpshooter (GWSS), Homalodisca coagulata (Say) (Hemiptera: Cicadellidae), has triggered a statewide control eVort. Management of GWSS will include biological control using resident and imported natural enemies. Currently, very little information is available on the role of generalist predators in suppression of GWSS eggs, nymphs ...

1979
Norman E Crook Christopher C Payne

Direct, indirect and double antibody sandwich methods of ELISA were examined for their ability to detect and discriminate between granulosis viruses from Pieris brassicae, Agrotis segetum and Cydia pomonella and for their specificity in the presence of host material. The indirect method was the most sensitive, capable of detecting down to about I ng of dissolved capsules/ml compared with IO ng/...

Journal: :Memorias do Instituto Oswaldo Cruz 1999
D E Rebeski E M Winger B Rogovic M M Robinson J R Crowther R H Dwinger

The diagnosis of trypanosomosis in animals with low parasitaemia is hampered by low diagnostic sensitivity of traditional detection methods. An immunodiagnostic method based on a direct sandwich enzyme-linked immunosorbent assay (ELISA), using monoclonal antibodies, has been examined in a number of African laboratories for its suitability for monitoring tsetse control and eradication programmes...

1998
James R. Hagler

Five different immunoassay formats were examined for their ability to detect a minute quantity of prey remains in predator guts. The convergent lady beetle, Hippodamia convergens Guérin-Meneville, that had consumed either one or five pink bollworm, Pectinophora gossypiella (Saunders), eggs was evaluated by the following immunoassays: three variations of enzyme-linked immunosorbent assay (ELISA)...

Journal: :Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 2009
Farida Ghalmi Bernard China Rachid Kaidi Bertrand Losson

Neospora caninum is a parasite responsible for abortion in cows and neuromuscular disease in dogs. Serology is the most widely used technique to evaluate the prevalence of N. caninum in different host populations. A sandwich enzyme-linked immunosorbent assay (ELISA) based on the use of an anti-SRS2 monoclonal antibody was evaluated against the indirect fluorescent antibody test for 100 canine s...

Journal: :Clinical and diagnostic laboratory immunology 1999
H Miyazawa H Bannai T Yanase C Morita S Satoh J Sugiyama S Taniguchi S Inouye

A reverse-sandwich enzyme-linked immunosorbent assay (ELISA), in which an antibody is sandwiched by antigens, was established for the titration of antibodies to verocytotoxins (VT) in human and animal sera. This assay has two advantages over a conventional indirect ELISA: (i) higher specificity and sensitivity and (ii) the ability to comparably titrate antibodies from different species. The VT1...

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