PURPOSE Here, we investigated the formation and functional properties of self-assembled lecithin/chitosan nanoparticles (L/C NPs) loaded with insulin following insulin-phospholipid complex preparation, with the aim of developing a method for oral insulin delivery. METHODS Using a modified solvent-injection method, insulin-loaded L/C NPs were obtained by combining insulin-phospholipid complexe...

This paper applies I.M. Gelfand’s distinction between adequate and non-adequate use of mathematical language in different contexts to the newly opened window of model-based measurements of intracellular dynamics. The specifics of geometry and dynamics on the mesoscale of cell physiology are elaborated in contrast to the familiar Newtonian mechanics and the more recent, but by now also rather we...

We describe the fabrication of a surface acoustic wave (SAW) atomizer, and show its ability to generate monodisperse aerosol and particles for drug delivery applications. In particular, we demonstrate the generation of insulin microdroplets for pulmonary delivery, and polymeric nanoparticles for one-step rapid encapsulation using SAW devices with resonance frequencies of 8.6 MHz and 19.3 MHz. I...

Metal catalyzed oxidation via the oxidative system Cu(2+)/ascorbate is known to induce aggregation of therapeutic proteins, resulting in enhanced immunogenicity. Hence, inclusion of antioxidants in protein formulations is of great interest. In this study, using recombinant human insulin (insulin) as a model, we investigated the ability of several excipients, in particular triethylenetetramine (...

CONTEXT Over the last few decades, extensive efforts have been made worldwide to develop nanomedicine delivery systems, especially via oral route for antidiabetic drugs. Absorption of insulin is hindered by epithelial cells of gastrointestinal tract, acidic gastric pH and digestive enzymes. EVIDENCE ACQUISITION Recent reports have identified and explained the beneficial role of several struct...

conclusions eudragit coated nanoparticle of chitosan is an efficient choice for oral delivery of dna to upper part of gi tract. results the mean size and zeta potential of particles were 300 ± 4 nm and 14 ± 0.5 mv, respectively. encapsulation of this system was 89.6 ± 1.2%. dna release from batches was less than 12% at ph = 5.2 and more than 60% at ph = 6.8 with significant difference of p < 0....