A reversed-phase system is described for the simultaneous isocratic separation of coproporphyrin I, II, III and IV isomers. The retention behaviour of coproporphyrin I and III is studied in detail. The method is suitable for both analytical and semi-preparative separation.

This Technical Overview demonstrates and discusses the influence of the injection parameters Feed Speed and Overfeed Volume on chromatographic separation. These parameters were introduced with the Agilent 1260 Infinity II Supercritical Fluid Chromatography (SFC) Multisampler. The influence on the isocratic separation of closely eluting compounds, shown for different injection volumes commonly u...

The presence of paralytic shellfish poisoning (PSP) toxins in cultures of Aphanizomenon flos-aquae, isolated from the Crestuma-Lever reservoir, was found by reversed phase high performance liquid chromatography employing two isocratic elution systems for the separation of PSP toxins. With the first isocratic elution protocol, the presence of apolar toxins as saxitoxin, decarbamoyl saxitoxin and...

A new isocratic HPLC method is optimized and validated for simultaneous determination of Vildagliptin (VLD), Pioglitazone Hydrochloride (PIO) and Glimepiride (GLIM) in Bulk and tablets (Gliptus ® and Amaglust ® tablets). The chromatographic separation was achieved on a reversed-phase analytical column [Hypersilgold ® C18 (10μm, 150 x 4.6 mm) column] at ambient temperature. The separation was ac...

The separation of dilute binary mixtures of proteins by salt aided ion-exchange simulated moving bed (SMB) chromatography is optimized with respect to throughput, desorbent consumption and salt consumption. The optimal flow-rate ratios are analytically determined via an adopted "triangle theory". Azeotropic phenomena are included in this procedure. The salt concentrations in the feed and recycl...

A two-step HPLC separation that combines isocratic and gradient elution has been developed for the analysis of trace impurities in fatty alcohol ethoxylates (FAEs). Quantitative estimates of the early eluting impurities were obtained from a calibration curve based on the responses of an evaporative light scattering detector (ELSD). Isocratic conditions were used to elute the impurities and grad...

A simple, selective and precise stabilityindicating HPLC method for determination of glabridin diacetate and dihexanoate prodrugs was developed, validated and applied to the enzymatic and chemical hydrolysis studies. The chromatographic separation was achieved on a reverse phase C18 (Thermo Hypersil-Keystone, 250 × 4.6 mm, 5 micron) column using the mixture of acetonitrile and water as mobile p...

This paper presents multi-objective optimization analysis and experimental implementation of a single column isocratic supercritical fluid chromatography process for the enantioseparation of flurbiprofen. The single column process is simulated using a detailed model with equilibrium description by a competitive Langmuir isotherm. The optimization problem has been formulated with the objectives ...