a major function of the enterocytes is absorption, processing, and export of dietary lipids into the lymphatic system. pluronic l-81 is a non-ionic hydrophobic surfactant, which specifically inhibits lipid absorption in the intestine when administered in vivo. this compound is therefore an attractive probe to perturb and investigate the molecular and intracellular events in chylomicron assembly...

Close intra-arterial infusion of the appetite regulating peptide orexin-A stimulates bicarbonate secretion from the duodenal mucosa. The aim of the present study was to elucidate the ability of orexin-A to induce intracellular calcium signaling in acutely isolated duodenal enterocytes. Freshly isolated clusters of enterocytes, obtained from rat duodenal mucosa or human duodenal biopsies, were l...

BACKGROUND/AIMS Acute stimulation by cAMP of the sodium dependent glucose cotransporter SGLT1 has previously been shown. As prostaglandin E2 (PGE2) increases intracellular cAMP concentrations via its receptor subtypes EP2R and EP4R, it was investigated whether PGE2 could enhance intestinal glucose absorption. METHODS The action of PGE2 on carbohydrate absorption in the ex situ perfused rat sm...

The use of a biomodel the confluent monolayer Caco-2 enterocytes allowed us to reveal ability S-protein isolated from strain LC2029 prevent permeability disorders resulting intestinal infections induced by pathogens (E. coli O157: H7, C. jejuni ATCC 33291, S. enteritidis 25928). This is important for maintaining efficiency barrier.

The aim of this study was to determine the intracellular events in chylomicron assembly in adult villus enterocytes. We have used novel methods for separation of the intracellular components of the secretory compartment [rough and smooth endoplasmic reticulum (RER and SER, respectively) and Golgi], and their membrane and luminal components, from villus enterocytes isolated from rabbit small int...

Isolated ileal enterocytes incubated with intrinsic factor-[57Co]vitamin B12 (IF-B12) at 37 degrees C took up 25 times more B12 than jejunal enterocytes. Uptake of B12 by ileal cells was dependent upon IF and Ca2+. B12 taken up by ileal enterocytes could be separated into two components: 1) B12 which was retained (E component) and 2) released (R-E component) by chelation of divalent cations. Th...

The increase in the prevalence of human obesity highlights the need to identify molecular and cellular mechanisms involved in control of feeding and energy balance. Oleoylethanolamide (OEA), an endogenous lipid produced primarily in the small intestine, has been identified to play an important role in the regulation of animal food intake and body weight. Previous studies indicated that OEA acti...