in this study, a new reaction system for quantitative determination of ascorbic acid was introduced. the developed method is based on inhibitory effect of ascorbic acid on the orange g-bromate system. the change in absorbance was followed spectrophotometrically at 478 nm. the dependence of sensitivity on the reaction variables including reagents concentration, temperature and time was investiga...

a sensitive and selective kinetic-spectrophotometric method was developed for the determination of trace amounts of uric acid. the method is based on the inhibitory effect of uric acid on the oxidation of janus green by bromate in the presence of sulfuric acid. the reaction was followed spectrophotometrically by measuring the absorbance at 518 nm using the fixed-time method. under optimum exper...

Several intermediates in the reaction of 2-methylglutamate with glutamate decarboxylase from Escherichia coli were detected by stopped-flow spectrophotometry and by rapid-scanning spectrophotometry after conventional mixing. Structures were assigned to intermediates on the basis of kinetic and spectral evidence. In the early stages of the reaction an intermediate with the properties expected of...

the kinetics and mechanism of the reaction between benzaldehyde 1, malononitrile 2 and dimedone 3 in a mixture of ethanol and water as solvents in the presence of fructose as a biodegradable catalyst has been studied by the spectroscopic method (uv-vis spectrophotometry method). the influence of various parameters (temperature, solvent and concentration) was studied on the reaction by means of ...