Background: Environmental noise is a known stressful factor that induces alterations of various physiological responses in the exposed individuals. It is known very little about the effect of prenatal noise stress on the serum glucose, lipids and morphology of Langerhans islets in neonatal rats. Objectives: In the present study the effect of chronic noise stress on hyperglycemia and lipid profi...

The small clusters of cells scattered in the pancreas and discovered by Langerhans in 1869, currently known as the islets of Langerhans, are at the centre of the pathology of diabetes. Today they appear as sophisticated micro-organs in which various cell types function in a remarkably co-ordinated manner. Until recently, most of the interest has been centred on the β-cells, which synthesise, st...

Isolation of rat islets of Langerhans was carried out by using collagenase and the destruction of exocrine tissue was performed through the injection of Hanks' solution into the common bile duct. The identification of the islets was done by techniques including the use of a stereomicroscope against a black background. Vital staining was done by an injection of neutral red solution through t...

In vitro research in the field of type I diabetes is frequently limited by the availability of a functional model for islets of Langerhans. This method shows that by the addition of theophylline to the glucose buffers, mouse insulinoma MIN6 and rat insulinoma INS1E pseudo-islets can serve as a model for islets of Langerhans for in vitro research. The effect of theophylline is dose- and cell lin...

We present a new technique for analyzing the three-dimensional structure of intact isolated islets of Langerhans. Adult rat and human islets were stained with whole-mount immunofluorescence techniques and optically sectioned with a confocal microscope. This has several advantages over traditional methods: 1) the technical difficulties in serial sectioning and handling the large numbers of secti...

Objective(s): Diabetes is a metabolic disease with an increasing prevalence for which finding new and efficient therapeutic approaches has always been a challenge. Preserving integrity and functionality of pancreatic β-cells as the only source of insulin in the body is such a case. To achieve this goal different cellular targets have been proposed among which pancreatic estrogen receptors have ...

The effect of Ca and calmodulin has been studied on adenylate cyclase activity in homogenates of rat islets of Langerhans. EGTA had a stimulatory effect on the enzyme in accord with the known inhibitory effect of Ca. In contrast, the addition of Ca together with calmodulin is stimulatory and demonstrates the existence of a Ca-dependent adenylate cyclase in islets of Langerhans. It is suggested ...

INTRODUCTION: WHO predicts an increase in the number of people with diabetes Indonesia from 8.4 million 2000 to around 21.3 2030. Another marker for measuring insulin resistance is homeostasis model assessment-insulin (HOMA-IR). Bariatric surgery most effective therapy patients both terms weight loss and improvement obesity-related diseases such as Type-2 Diabetes Mellitus (DM). OBJECTIVE: This...

Beta-cells inside the pancreatic islets of Langerhans are the only source of the hormone insulin. Visualization of pancreatic islets with MRI in the mouse has many potential applications in preclinical diabetes research, because the amount of beta-cells decreases with the onset of the disease. Islets of Langerhans were imaged with a beta-cell specific MRI contrast agent in the excised murine pa...

OBJECTIVE Ganoderma Lucidum (G. Lucidum) has been suggested to increase serum insulin level. This study was undertaken to investigate its direct effect on the islets of Langerhans. MATERIAL AND METHODS Male albino Wistar rats were anesthetized and the islets were isolated after digestion of the pancreas with collagenase. The islets were incubated for 60 min in Krebs bicarbonate buffer contain...