background: leishmaniasis is a worldwide disease prevalent in tropical and sub tropical coun­tries. many attempts have been made and different strategies have been approached to develop a potent vaccine against leishmania . dna immunisation is a method, which is shown to be effec­tive in leishmania vaccination. leishmania soluble antigen (sla) has also recently been used leishmania vaccination....

Dogs play an important role in transmission of Leishmania infantum, but epidemiologic and clinical studies canine tegumentary leishmaniasis (CTL) are scarce. In endemic area human American (ATL) caused by braziliensis, we determine the prevalence incidence both CTL subclinical (SC) L. braziliensis infection dogs evaluated if presence with or SC is associated occurrence ATL. healthy animals ulce...

Background: Leishmania is a pathogenic parasite which infects mononuclear cells in vertebrate hosts. Different strategies have been taken to develop immunity against Leishmania . DCs loaded with immunogenic antigen have resulted in different levels of Th1-type immune response and cytotoxic T lymphocytes (CTL) activity. Objective: To evaluate the potency of DCs primed with soluble Leishmania mex...

Infection with Leishmania infantum causes the disease visceral leishmaniasis (VL), which is a serious clinical and veterinary problem. The drugs used to treat canine (CanL) do not cause complete parasite clearance; they can be toxic, emerging drug resistance in populations limits their utility. Therefore, this study we have evaluated toxicity efficacy of joint treatment 1:1 mixture sodium stibo...

INTRODUCTION To determine the immunostimulatory potential of crude extracts of Warburgia ugandensis subsp. ugandensis with a soluble leishmanial antigen in vaccinating BALB/c mice. METHODS Seventy two female BALB/c mice were randomly assigned into six groups. The mice were vaccinated with soluble leishmania antigens (SLA) alone, hexane, ethyl acetate, and dichloromethane extract co-administer...

The Leishmania homologue of activated C kinase (LACK) a known T cell epitope from soluble Leishmania antigens (SLA) that confers protection against Leishmania challenge. This antigen has been found to be highly conserved among Leishmania strains. LACK has been shown to be protective against L. donovani challenge. A comprehensive analysis of several LACK sequences was completed. The analysis sho...

Background: Monoclonal antibody technology allows identification of amastigote-specific antigen in human tissue biopsies and search for a suitable vaccine candidate, which may induce a long lasting immunity. Objectives: Production and partial characterization of seven monoclonal antibodies against Leishmania tropica promstigotes. Methods: Seven murine monoclonal antibodies of the IgG isotype we...

Delayed-type hypersensitivity reaction measured by skin testing, is an important tool for evaluation of cellular immune response in leishmaniasis and has been used recently as an indication of exposure to infection. Skin testing in leishmania infection needs a standard reagent with high specificity, sen‌sitivity, and potency. In the present work, a soluble leishmanin, and three whole parasite p...

BACKGROUND In areas endemic for visceral leishmaniasis (VL), a large number of infected individuals mount a protective cellular immune response and remain asymptomatic carriers. We propose an interferon-gamma release assay (IFN-γRA) as a novel marker for latent L. donovani infection. METHODS AND FINDINGS We modified a commercial kit (QuantiFERON) evaluating five different leishmania-specific ...

delayed-type hypersensitivity reaction measured by skin testing, is an important tool for evaluation of cellular immune response in leishmaniasis and has been used recently as an indication of exposure to infection. skin testing in leishmania infection needs a standard reagent with high specificity, sen sitivity, and potency. in the present work, a soluble leishmanin, and three whole parasite p...