background: direct identification of leishmania species in giemsa-stained slides without parasite culturing in the areas where multiple species exist, is very helpful. this study was designed to isolate leishmani spp. from giemsa-stained smears and to characterize them by pcr technique. methods: a total of 48 giemsa-stained slides from confirmed cases of leishmaniasis were examined under a ligh...

in this study, we used random amplified polymorphic dna (rapd) for identification of 17 isolates of leishmania from the skin and reticuloendothelial system of humans, animal reservoirs (rodent and dog) and sandflies in various parts of iran in the last decade. fifteen species have been confirmed by isoenzyme characterization by the london school of hygiene and tropical medicine and shiraz unive...

Background: Lipophosphoglycan 3 (LPG3) is required for the LPG assembly, a well known virulent molecule. In this study, the LPG3 gene of the lizard and mammalian Leishmania species were cloned and sequenced. A three-dimensional structure (3D) for the target sequence was also predicted by comparative (homology) modeling. Materials and Methods: An optimization PCR amplification was performed o...

both zoonotic and anthroponotic cutaneous leishmaniasis (cl) caused by l. major and l. tropica, respectively, are endemic in different parts of iran. this study was performed to investigate the new changes in epidemiological pattern of cl, and to identify the species of leishmania and the strains of l. major isolated from different endemic areas of iran. seventy-two isolates from 245 samples co...

The Leishmania species cause a variety of human disease syndromes. Methods for diagnosis and species differentiation are insensitive and many require invasive sampling. Although quantitative PCR (qPCR) methods are reported for leishmania detection, no systematic method to quantify parasites and determine the species in clinical specimens is established. We developed a serial qPCR strategy to id...

The in vitro leishmanicidal activity of miltefosine® (Zentaris GmbH) was assessed against four medically relevant Leishmania species of Brazil: Leishmania (Leishmania) amazonensis, Leishmania (Viannia) braziliensis, Leishmania (Viannia) guyanensis and Leishmania (Leishmania) chagasi. The activity of miltefosine against these New World species was compared to its activity against the Old World s...

background: cutaneous leishmaniasis (cl) is a major health problem in many parts of iran, although diagnosis of cl especially in the endemic area is easy, but treatment and management of the disease is a global dilemma. diag-nosis of cl in non-endemic area is not as simple as in endemic foci. in this study, the status and the proportions of cl induced by leishmania major and l. tropica among cl...

background: leishmaniasis is endemic in iran. different species of leishmania (l.) parasites are causative agents of this disease. correct identification of leishmania species is important for clinical studies, prevention, and control of the diseases. mix up of leishmania isolates is possible in the laboratory, so there is need for verification of species for isolates of uncertain identity. dif...