We examined some of the metabolic events that regulate sensitizing cancer extract-induced leukocyte adherence inhibition and found that human leukocytes adhere in a comparatively passive manner to glass in serum-free medium. Adherence of leukocytes to glass did not require oxidative metabolism, microtubules, microfilaments, or calcium entry, whereas leukocyte mobility excited by sensitizing can...

We demonstrate an additional step and a positive feedback loop in leukocyte accumulation on inflamed endothelium. Leukocytes in shear flow bind to adherent leukocytes through L-selectin/ligand interactions and subsequently bind downstream and roll on inflamed endothelium, purified E-selectin, P-selectin, L-selectin, VCAM-1, or peripheral node addressin. Thus adherent leukocytes nucleate formati...

PURPOSE We reviewed various methodologies for studying leukocyte-retinal endothelial interactions in vivo, and summarized the information obtained from studies employing these methods. PROCEDURES Fluorescence dye-enhanced scanning laser ophthalmoscopy facilitates study of leukocyte-cell interactions in the retinal microcirculation. RESULTS Various methods such as adaptive optics scanning la...

OBJECTIVE To study the role of leukocyte count in patients with unstable angina pectoris or myocardial infarction (Acute Coronary Syndrome) its prognostic significance and correlation with plasma brain natriuretic peptide (BNP) levels. METHODS A total of 143 Patients with unstable angina pectoris, non-ST segment elevation MI and ST segment elevation MI were considered for entry into the study...

The migration of leukocytes from the vascular lumen to sites of infection and/or injury in the extravascular tissue involves a series of sequential and coordinated molecular and cellular events with the resultant primary response being that of reduced leukocyte velocity within the blood stream, followed by leukocyte firm adhesion to endothelial cells lining the vessel wall and eventually migrat...

Background: Coronary artery diseases (CAD) is the most common cause of death in the developing counteries.  Atherosclerosis is the leading cause of CAD. A number of studies have shown that total leukocyte count is a risk factor for CAD but there is little information on the role of eosinophilia and allergy.  The aim of this study was to determine the relationship between eosinophilia and histor...

A new method is described for the quantitative study of leucocyte migration. Blood drawn into a capillary tube is centrifuged, forming a buffy coat. The migration of the leucocytes from the buffy coat into the plasma is measured by means of an ocular micrometer. While there is a great deal of variation from one normal individual to another in the migration rates of leucocytes those of each indi...

Thrombosis and inflammation involve complex platelet-leukocyte interaction, the details of which are not fully elucidated. Therefore, we investigated cross talk between platelets and leukocytes in whole blood, under the following physiological conditions: at 37 degrees C, with normal calcium concentrations, and with shear force. Platelet P-selectin and leukocyte CD11b expression were used to mo...

The incidence and characteristics of HLA alloimmunization following transfusions of leukocyte concentrates as a source of enzyme replacement were determined in male patients with Hunter's syndrome. Five patients were given leukocyte concentrates from HLA matched donors (Group I) and another five patients received leukocyte concentrates from non-HLA matched donors (Group II). No other blood prod...

Cells migrating within tissues may encounter multiple chemoattractant signals in complex spatial and temporal patterns. To understand leukocyte navigation in such settings, we have explored the migratory behavior of neutrophils in model scenarios where they are presented with two chemoattractant sources in various configurations. We show that, over a wide range of conditions, neutrophils can mi...