background: this study quantified the effect of four popular culture media in a biodiesel production study on the qualitative and quantitative lipid content, dry biomass, and lipid productivity of nannochloropsis oculata. methods: culture of microalgae was done separately in walne, f/2, sato, and tmrl media. in the logarithmic and stationary growth phases, biomass production and lipid accumulat...

Nitrogen starvation is an efficient environmental pressure for increasing lipid accumulation in microalgae, but it could also significantly lower the biomass productivity, resulting in lower lipid productivity. In this study, green alga Chlorella sp. A2 was cultivated by using a minimal nitrogen supply strategy under both laboratory and outdoor cultivation conditions to evaluate biomass accumul...

BACKGROUND Industrial biotechnology that is able to provide environmentally friendly bio-based products has attracted more attention in replacing petroleum-based industries. Currently, most of the carbon sources used for fermentation-based bioprocesses are obtained from agricultural commodities that are used as foodstuff for human beings. Lignocellulose-derived sugars as the non-food, green, an...

Lab scale studies were conducted in a customized photobioreactor to assess the lipid productivity of Chlorella pyrenoidosa under fed-batch mode using 0.025g/l to 0.1 g/l urea and sodium nitrate as nitrogen source in nutrient medium. The light intensities used were 100 μmol ms and 135 μmol ms. The results indicated maximum lipid productivity of 0.103 g/d.l in BG11 medium with 0.1g/l sodium nitra...

BACKGROUND Microalgal oil is a promising alternative feedstock for biodiesel fuel (BDF). Mixotrophic cultivation with glycerol, the primary byproduct of BDF production, may be used to optimize BDF production. This strategy would reduce costs through glycerol recycling and improve lipid productivity and biomass productivity by overcoming the growth retardation caused by decreased light penetrati...

Background: This study quantified the effect of four popular culture media in a biodiesel production study on the qualitative and quantitative lipid content, dry biomass, and lipid productivity of Nannochloropsis oculata. Methods: Culture of microalgae was done separately in Walne, F/2, Sato, and TMRL media. In the logarithmic and stationary growth phases, biomass production and lipid accumulat...

Five non-toxin producing cyanobacterial isolates from the genera Synechococcus, Trichormus, Microcystis, Leptolyngbya and Chlorogloea were examined in terms of quantity and quality as lipid feedstock for biofuel production. Under the conditions used in this study, the biomass productivity ranged from 3.7 to 52.7 mg·L-1·day-1 in relation to dry biomass, while the lipid productivity varied betwee...

micro-organisms such as bacteria, yeasts, molds and algae that accumulate lipid more than 20% of their biomass are called oleaginous. microbial lipid has a lot of similarity to the oil obtained from plants and animals. microbial lipids are renewable sources that can use for different purposes such as biodiesel production. production of oil from yeasts has more advantages than plant’s oil. accor...

Influence of mixotrophic mode and its transition to various trophic modes under stress conditions was assessed during two stage cultivation of Chlorella pyrenoidosa. Significant lipid productivity was triggered under low light intensity, glucose + bicarbonate supplementation and nitrogen starvation. The association between biomass and lipid productivity, fatty acid composition during mixotrophi...

The oleaginous microalga Chlorella sp. LAM-H screened from freshwater was proven to be a prospective feedstock for oil production according to its fatty acid composition. In order to enhance lipid production, response surface methodology (RSM) was used with central composite design (CCD) to optimize the heterotrophic cultivation of microalgae. The experiment results showed that a satisfactory s...