Human manganese superoxide dismutase is a mitochondrial metalloenzyme that is involved in protecting aerobic organisms against superoxide toxicity, and has been implicated in slowing tumor growth. Unfortunately, this enzyme exhibits strong product inhibition, which limits its potential biomedical applications. Previous efforts to alleviate human manganese superoxide dismutase product inhibition...

A mouse fibrosarcoma cell line (FSa-II), which exhibits low endogenous levels of manganese Superoxide dismutase, was transfected with a human manganese Superoxide dismutase complementary DNA. Fifty clones were screened for manganese Superoxide dismutase activity by the Superoxide dismutase activity gel assay. Activity of the positive clones was measured by the nitro blue tetrazolium-reduction a...

A mouse fibrosarcoma cell line (FSa-II), which exhibits low endogenous levels of manganese superoxide dismutase, was transfected with a human manganese superoxide dismutase complementary DNA. Fifty clones were screened for manganese superoxide dismutase activity by the superoxide dismutase activity gel assay. Activity of the positive clones was measured by the nitro blue tetrazolium-reduction a...

BACKGROUND AND AIMS Excessive production of reactive oxygen metabolites (ROMs) by phagocytic cells is thought to contribute to the mucosal pathology of Helicobacter pylori infection. Previously, H pylori infection was shown to have a differential effect on some gastric mucosal scavenger enzymes of ROMs-namely, mitochondrial and cytoplasmic superoxide dismutases-reflected by a large increase in ...

Transfection of a human pSV2 (copper-zinc) superoxide dismutase expression vector into murine fibroblasts resulted in stable clones producing increased amounts of copper-zinc superoxide dismutase. A marked increase in endogenous glutathione peroxidase activity (up to 285%) and a smaller increase in glutathione transferase activity (up to 16%) also occurred. Manganese superoxide dismutase activi...

Dopaminergic neurons of the substantia nigra are susceptible to toxin-based insults. Intrastriatal injection of 6-hydroxydopamine results in selective toxicity to these neurons. A mechanistic role for reactive oxygen species is supported by observations that antioxidants confer protection from 6-hydroxydopamine. Although cell culture studies have suggested extracellular or nonmitochondrial mech...

2 Introduction 3 Phylogenetic distribution and subcellular localization 3 Kinetics and mechanism 3 Molecular and chemical structure 5 Biological significance 8 Summary 9 References 10 Abstract Manganese superoxide dismutase (MnSOD) is the primary antioxidant enzyme that protects cells from oxidative stress by catalyzing dismutation of superoxide (O2 ) to hydrogen peroxide and oxygen in the mito...

patients and methods manganese superoxide dismutase polymorphism was genotyped using polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) in 103 patients with mlds and 103 healthy control subjects. conclusions according to the fact that mn-sod gene polymorphisms have been considered as a major risk factor in some malignancies, this single center study did not find any a...

The major superoxide dismutase of the unicellular red alga, Porphyridium cruentum, has been purified to homogeneity. This enzyme has a molecular weight of 40,000 and is composed of two subunits of equal size, which are joined by noncovalent interactions. Manganese constituted 0.13% of this superoxide dismutase. T,is is equivalent to 1 manganese atom/molecule of enzyme. Cyanide at 5 mM and H2O2 ...

The anaerobic, but aerotolerant Propionibacterium freudenreichii sp. shermanii contains a single superoxide dismutase [EC 1.15.1.1.] exhibiting comparable activity with iron or manganese as metal cofactor. The formation of superoxide dismutase is not depending on the supplementation of iron or manganese to the culture medium. Even in the absence of these metals the protein is built in comparabl...