backgrounds: evidence is accumulating to support disruption of tissue architecture as a powerful event in tumor formation. for the past four decades, intensive cancer research with the premise of “cancer as a cell based-disease” focused on finding oncogenes or tumor suppressor genes. however, the role of the tissue architecture was neglected. three dimensional (3d) cell cultures which can recap...

the ability to discover services in mobile ad hoc network (manet) is a major prerequisite. cluster basedcross layer intelligent service discovery for manet (cbisd) is cluster based architecture, caching ofsemantic details of services and intelligent forwarding using network layer mechanisms. the cluster basedarchitecture using semantic knowledge provides scalability and accuracy. also, the mini...

The steam reforming of ethanol (SRE) is a key process for the production H2 and other vital hydrocarbons. present work describes synthesis Platinum-Gallium (Pt−Ga) nanoalloys supported on mesostructured cellular foam (MCF-17) via ultrasound-assisted impregnation method. Ga was substituted with Pt in different wt.% i. e. Pt/MCF-17, Pt99.9Ga0.1/MCF-17, Pt99Ga1/MCF-17, Pt90Ga10/MCF-17 evaluated to...

Dielectrophoresis (DEP) was used to examine a panel of MCF-7 cell lines comprising MDR derivatives: MCF-7TaxR (paclitaxel –resistant, P-glycoprotein (P-gp) positive), MCF-7DoxR (doxorubicin-resistant P-gp negative) plus MCF-7MDR1 (MDR1 transfected, P-gp positive). MCF-7DoxR and MCF-7MDR1 were broadly cross-resistant to natural product anticancer agents, whereas MCF-7TaxR cells were not, contrar...

We explored whether p53 upregulated modulator of apoptosis (PUMA) gene transfection could enhance the sensitivity of epirubicin-induced apoptosis of MCF-7 breast cancer cells. The liposome-mediated recombinant eukaryotic expression vector PU-MA-pCDNA3 and empty vector plasmid were stably transfected into MCF-7 cells. Epirubicin (0.01-100 μM) was applied to MCF-7, MCF-7/PUMA, and MCF-7/pCDNA3 ce...

We have studied the effect of selenium on the expression of a cellular glutathione peroxidase, GSHPx-1, in transfected MCF-7 cells and in doxorubicin-resistant (Adrr) MCF-7 cells. A GSHPx-1 cDNA with a Rous Sarcoma virus promoter was transfected into a human mammary carcinoma cell line, MCF-7, which has very low endogenous cytosolic glutathione (GSH) peroxidase activity and no detectable messag...

Backgrounds: Evidence is accumulating to support disruption of tissue architecture as a powerful event in tumor formation. For the past four decades, intensive cancer research with the premise of “cancer as a cell based-disease” focused on finding oncogenes or tumor suppressor genes. However, the role of the tissue architecture was neglected. Three dimensional (3D) cell cultures which can recap...

conclusions these outcomes support our theory that rapamycin increases oxidative stress in mcf-7 and mda mb-231 cells but also shows high levels of antioxidant effects, which probably limit the effects of the rapamycin on the same issue in the clinic. methods the mcf-7 and mda mb-231 cell lines were cultivated and treated with rapamycin for 72 hours. the viability of the cells was determined us...