Dbf4-dependent kinases (DDKs) are required for the initiation of DNA replication, their essential targets being the MCM2-7 proteins. We show that, in Xenopus laevis egg extracts and human cells, hyper-phosphorylation of DNA-bound Mcm4, but not phosphorylation of Mcm2, correlates with DNA replication. These phosphorylations are differentially affected by the DDK inhibitors PHA-767491 and XL413. ...

Propylene is an important raw material for the production of many valuable compounds, especially polypropylene, consumption which continues to grow every year. The reaction oxidative dehydrogenation propane, where carbon dioxide used as a mild oxidant, promising method producing propylene. At same time, problem utilization greenhouse gas CO2 partially solved. synthesis and analysis physicochemi...

Catalytic distillation is a technology that combines heterogeneous catalytic reaction and the separation of reactants products via in single reactor/distillation system. This process catalysis, kinetics, mass transfer to obtain more selective products. The catalyst provides sites for reactions porous surface liquid/vapor separation. advantages are energy savings, low waste streams, longevity, h...

We report a multi-mode compact microscope (MCM) for high-contrast and high-resolution imaging. The MCM consists of two LED illuminations, magnification lens, lift stage, housing with image processing control boards. allows multi-modal imaging, including reflection, transmission, higher modes. dual illuminations also provide imaging various targets such as biological samples microcircuits. high ...

VO2+ and its complexes with ethylenediamine (en), acetylacetonate (acac), 2, 2’-bipyridine(bpy) ligands, immobilized within nanoreactors of Al-MCM-41 designated as VO2+/Al-MCM-41 or VOL2/Al-MCM-41, were prepared and characterized by X-ray powder diffraction (XRD), FT-IR, BET nitrogen adsorption-desorption and chemical analysis techniques. VO2+/Al-MCM-41 and VOL2/Al-MCM-41 were found to catalyze...

Replicating large eukaryotic genomes presents the challenge of distinguishing replicated regions of DNA from unreplicated DNA. A heterohexamer of minichromosome maintenance (MCM) proteins is essential for the initiation of DNA replication. MCM proteins are loaded on to unreplicated DNA before replication begins and displaced progressively during replication. Thus, bound MCM proteins license DNA...

Minichromosome maintenance (MCM) proteins play an essential role in DNA unwinding and are completely conserved throughout eukaryotic and archaeal cells, underscoring their fundamental importance in DNA replication. Biochemists have characterized some of the protein motifs that affect the unwinding of DNA by MCM proteins. Elizabeth Jenkinson and James Chong studied how changes in the MCM protein...

In animals, cobalamin (Cbl) is a cofactor for methionine synthase and methylmalonyl-CoA mutase (MCM), which utilizes methylcobalamin and 5'-deoxyadenosylcobalamin (AdoCbl), respectively. The cblA complementation class of inborn errors of Cbl metabolism in humans is one of three known disorders that affect AdoCbl synthesis. The gene responsible for cblA has been identified in humans (MMAA) as we...

The minichromosome maintenance complex (MCM) represents the replicative DNA helicase both in eukaryotes and archaea. Here, we describe the solution structure of the C-terminal domains of the archaeal MCMs of Sulfolobus solfataricus (Sso) and Methanothermobacter thermautotrophicus (Mth). Those domains consist of a structurally conserved truncated winged helix (WH) domain lacking the two typical ...