abstract background: improvements in culture media formulations have led to an increase in the ability of sheep embryo in culture throughout the preimplantation period. objective: this study was carried out to evaluate the effects of various concentrations of mem vitamins during in vitro maturation of sheep oocytes and subsequent embryo development. materials and methods: sheep ovaries were col...

BACKGROUND Improvements in culture media formulations have led to an increase in the ability of sheep embryo in culture throughout the preimplantation period. OBJECTIVE This study was carried out to evaluate the effects of various concentrations of MEM vitamins during in vitro maturation of sheep oocytes and subsequent embryo development. MATERIALS AND METHODS Sheep ovaries were collected f...

Due to the complicated media used for culturing bovine embryos, most of the nutrient requirements are unknown. Recently, we developed a simple, serum-free medium (CR1) that allows bovine embryos to develop in vitro. Therefore, our objective was to determine whether development of bovine embryos would be improved by the addition of free amino acids and vitamins to CR1. Oocytes were recovered fro...

Human embryonic diploid lung fibroblasts were capable of growing in a medium consisting of Eagle's MEM vitamins plus 0 1 /tg/ml biotin, Earle's salt solution, 4 mM glutamine, 0 4 raM cystine, 0-5 mM tyrosine, 10 % undialysed calf serum and 1 mM pyruvate. The growth rate and percent cell attachment in this medium was slightly less than in Eagle's MEM but the longevity was about the same. Additio...

We propose that elevation of mitochondrial enzyme cofactors may prevent or ameliorate neurodegenerative diseases by improving mitochondrial function. In the present study, we investigated the effects of high doses of B vitamins, the precursors of mitochondrial enzyme cofactors, on mitochondrial dysfunction, oxidative stress, and Parkinsonism in a 4-week long rotenone treatment-induced cellular ...

A family of mem-models, including the mem-dashpots, mem-springs, and most recently, mem-inerters, is emerging as a new powerful way capturing complex nonlinear behaviors materials systems under various types dynamic loads involving different frequency, amplitude, loading histories (e.g., hysteresis). Under framework state-space representation hybrid dynamical systems, mem-springs may be formula...

Homologous serum, when repeatedly used for the culture of postimplantation rat embryos, rapidly loses its capacity to support growth and development. Replenishment of the 'exhausted' serum with glucose and vitamins (MEM vitamin concentrate--Flow Laboratories) together with gentle dialysis to remove small molecular weight toxic metabolites (lactate etc) fails to restore the growth-promoting prop...