The bulk of the intracellular arginine pool in exponentially growing mycelia of Neurospora crassa is sequestered in the vacuoles. Vacuolar arginine effluxes from the vacuoles into the cytosol and is catabolized to ornithine and urea upon nitrogen starvation. The energy requirement for mobilization has been studied by treating nitrogen-starved mycelia with inhibitors or respiration or glycolysis...

The energy requirements for the uptake and retention of arginine by vacuoles of Neurospora crassa have been studied. Exponentially growing mycelial cultures were treated with inhibitors of respiration or glycolysis or an uncoupler of respiration. Catabolism of arginine was monitored as urea production in urease-less strains. The rationale was that the rate and extent of such catabolism was indi...

Biological inhibition of air pollution has vast advantages over physicochemical methods. One of the biggest challenges faced by researchers with traditional bio-filter in controlling Volatile Organic Compounds (VOCs) such as Benzene, Toluene, Ethylbenzene, and Xylene (BTEX) is, low degradation rate (elimination capacity) and accumulation of very high biomass. The use of metabolic uncouplers...

1. Pigeon heart mitochondria produce H(2)O(2) at a maximal rate of about 20nmol/min per mg of protein. 2. Succinate-glutamate and malate-glutamate are substrates which are able to support maximal H(2)O(2) production rates. With malate-glutamate, H(2)O(2) formation is sensitive to rotenone. Endogenous substrate, octanoate, stearoyl-CoA and palmitoyl-carnitine are by far less efficient substrates...

High concentrations of uncouplers of oxidative phosphorylation inhibit the oxidation of succinate and NADH catalyzed by phosphorylating beef heart submitochondrial particles. Inhibition is reversed by bovine serum albumin but not by ATP. ATP does not influence either the stimulation of respiration at low uncoupler concentrations or the inhibition of respiration at high uncoupler concentrations....

possible inhibition of absorption by folic acid as it interfered with the PAB assay. We conclude that PAB passes across human buccal mucosa by a non-saturable, Na+ -independent process presumably physical diffusion. These transport characteristics of PAB are similar to those found with small intestine of rats and hamsters in vitro (Spencer et al., 1966; Arvanitakis, 1978). PAB will be 92% ioniz...