A flow cytometric assay for lymphocyte HLA-B27 expression using a two-color direct immunofluorescent assay was compared to traditional microlymphocytotoxicity testing on 209 clinical samples. For the flow cytometric assay, whole blood was mixed with a monoclonal anti-B27 conjugated to fluorescein-isothiocyanate (FITC) and anti-CD3 conjugated to phycoerythrin (PE). The samples were analyzed with...

the human major histocompatibility complex (mhc) plays a crucial role in transplantation, transfusion, paternity test and assessment of susceptibility to some diseases associated with hla-b27. three of the most fashionable methods for determination of hla antigens in clinical and research laboratories are microlymphocytotoxicity (mlct), flowcytometry and polymerase chain reaction (pcr). the pur...

To determine whether genetic factors could be involved in the pathogenesis of rheumatic heart disease, we performed HLA-A and HLA-B typing in 59 Indian patients with severe chronic rheumatic heart disease requiring cardiac surgery, and HLA-DR and HLA-DQ typing in 58 of these patients. The HLA typing was done by a standard microlymphocytotoxicity method. Patients were 12 to 59 years old (mean 32...

Fifty consecutive patients with migraine headaches were evaluated for the presence of 10 HLA-A and 14 HLA-B antigens by means of the microlymphocytotoxicity assay. Comparisons were made between classical and common migraine, and between patients with and without a family history of migraine. All groups of patients showed an association with B8 and B27, which failed to reach clinical significanc...

DNA based HLA typing is fast replacing conventional Microlymphocytotoxicity based method, which has been regarded as the gold standard. Many laboratories in Mumbai have already switched over to molecular methods, as the results are far superior. This study was undertaken to compare the results of generic molecular typing by sequence specific primers (SSP) with that by serology in our laboratory...

OBJECTIVE This case-control study was undertaken to evaluate the association of HLA-B51 antigen and its allelic types with Behçet's disease (BD) and with recurrent aphthous stomatitis (RAS), to investigate the degree of this association with diagnostic types and clinical variables of BD. METHODS The DNA typing of HLA-B51 by nested PCR-SSP was performed in 61 patients with BD, 56 patients with...

Four platelet compatibility assays were performed on serum and platelet or lymphocyte samples from 38 closely HLA-matched donor/recipient pairs involved in 55 single-donor platelet transfusions. The 22 patients studied were refractory to transfusions of pooled random-donor platelets. Of the four assays (platelet suspension immunofluorescence, PSIFT; 51Cr release; microlymphocytotoxicity; and a ...

To determine whether genetic factors could be involved in the pathogenesis of rheumatic heart disease, we performed HLA-A and HLA-B typing in 120 black patients with severe chronic rheumatic heart disease requiring cardiac surgery, and HLA-DR and HLA-DQ typing in 103 and 97 of these patients, respectively. The HLA typing was done by a standard microlymphocytotoxicity method. Patients were 12 to...

The HLA (Human Leucocyte Antigens) class I and class II polymorphism in a sample population of 250 Vojvodinians using standard microlymphocytotoxicity test was investigated. The antigens with the highest frequencies were: HLA-A2 (50.4%), A1 (24.1%) and A3 (22.7%); B35 (22.7%), B51(5) (16.9%) and B18 (15.6%); DR11 (5) (34.5%), DR7 (20.0%) and DR3 (19.3%); DQ1 (44.8%), DQ7 (3) (30.9%) and DQ2 (13...