نتایج جستجو برای: microsatellite polymorphisms
تعداد نتایج: 84525 فیلتر نتایج به سال:
optimization of the condition for pcr-directed sequencing of microsatellites poly adenine (a) length polymorphisms is more difficult and sensitive compared with other common sequences. replication slippage may occur for polymerase enzyme during microsatellite amplification and direct sequencing of these pcr products will be challenging for heterozygote samples. so, the aim of this study is to i...
Development of microsatellite markers has been an increasing trend in crop genetic studies because oftheir applicability in breeding programs. Here we report the development of inter simple sequencerepeat (SSRs) in pomegranate (Punica granatum L.) using an enrichment method that makes use of magneticbeads. Enriched genomic libraries with AG and ATG microsatellite motifs were c...
The aims of the study were to detect the polymorphisms of three microsatellite sites: S11, S15, and S24 in the ovine PRNP and to asses their relationship with prion protein (PrP) genotypes in three sheep breeds. To identify 15 PrP genotypes based on polymorphisms at codons 136, 154, and 171, PCR-RFLP analyses were applied. The microsatellite sites were amplified. For each microsatellite two or ...
aim : the propose of this study was to evaluate the probable correlation between exon and intron polymorphisms of p53 gene and their association with clinicopathological aspects of gastritis. background : regarding to the decisive role of p53 in the development of a variety of human cancers, a comprehensive study concerning probable correlation between polymorphisms in the p53 intron and exon i...
Vol. 36, No. 3 (2004) BioTechniques 409 The process of microsatellite development and profiling involves three primary steps: (i) isolating regions of genomic DNA that contain microsatellite loci; (ii) developing strategies for screening each locus, which requires designing primers for amplification, optimizing reaction conditions, and screening for variation; and (iii) genotyping sampled indiv...
Disease-specific markers are critical for early diagnosis, targeted therapy and prognostic prediction of diseases. Current study reports a complex microsatellite as a new prognostic marker of sporadic colorectal cancer. This microsatellite located at Chromosome 7q33 is composed of three tetranucleotide tandem repeats, (TTCC)2(TCCC)5(TCCT)7, flanked by a CT-rich sequence. We analyzed polymorphis...
BACKGROUND The genetics involved in Ewing sarcoma susceptibility and prognosis are poorly understood. EWS/FLI and related EWS/ETS chimeras upregulate numerous gene targets via promoter-based GGAA-microsatellite response elements. These microsatellites are highly polymorphic in humans, and preliminary evidence suggests EWS/FLI-mediated gene expression is highly dependent on the number of GGAA mo...
BACKGROUND/AIMS Cortisol plays an important role during pregnancy. It controls maternal glucose metabolism and fetal development. Cortisol metabolism is partially controlled by the 11b-HSD2. This enzyme is expressed in the kidney and human placenta. The activity of the enzyme is partially controlled by functional polymorphisms: the HSD11B2[CA]n microsatellite polymorphism. The impact of this fu...
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