conclusions this study showed m. globosa to be the most common malassezia species isolated from malassezia skin disorders in kashan, iran. the pcr-rflp method was useful in the rapid identification of the malassezia species. by using these methods, the detection and identification of individual malassezia species from clinical samples was substantially easier. patients and methods a total of 14...

currently, different highly sensitive nucleic acid detection-based protocols used in medical diagnostic laboratories need specific working conditions, and if no optimization is performed, misleading results can be obtained. limitations of each protocol is different in obtaining an appropriate result. awareness of these limitations plays an important role in providing a reliable result for physi...

background nucleic acid based detection of mycobacterium tuberculosis has the potential to improve the analysis of the tuberculosis epidemiology and patient care by increasing the specificity and sensitivity of diagnosis. patients and methods fifty suspected cases were screened for m. tuberculosis by using a new primer sequence and comparing its results with the primer (is6110) most widely used...

leptospirosis is an acute infectious, systemic and septisemic disease which had recent outbreaks in some parts of iran especially in north provinces. rapid detection is a critical step for treatment and control of this disease. in this research a pcr based method was evaluated for detection of iranian local endemic serovars. all reported endemic serovars of leptospira including leptospira gripp...

background and objectives: a new real- time pcr protocol for the detection of trichomonas vaginalis in pooled urine samples has been optimized and validated. materials and methods: the amplification protocol, targeting a 2kb repeated gene in the t. vaginalis genome, was optimized by varying pcr parameters. as a reference method, a real-time pcr protocol targeting the beta-tubulin gene (y. versl...

background: diagnosis of some diseases is difficult due to invasive sampling. urine has been candi-date as a non-invasive and convenient alternative. it has many advantages and easy accessibility but some technical ills should be removed. finding a suitable extraction method for improving urine dna quantity and quality in altering invasive specimens for molecular diagnosis of some infectious di...

context: detection of causative pathogen is crucial for treatment strategies in most infections. molecular diagnosis as a more sensitive and specific method, has become the best option to investigate certain pathogens, mainly viruses. recent scientific and technological approaches resulted in changing the area of diagnostic microbiology. evidence acquisition: a comprehensive online search of da...

viral symptoms indicative of iris yellow spot virus (iysv) were observed on onion in several fields near chenaran in khorasan razavi province. mechanical inoculation of herbaceous hosts with onion sap extracts from symptomatic plants showed similar symptoms to those described for iysv. the mechanically transmitted virus reacted only with antisera specific to iysv in das-elisa but not with antis...

background: the aim of this study was to determine the prevalence of the nosema ceranae and nosema apis among apiaries using both spore counts and multiplex pcr and the replacement of n. apis by n. ceranae in some regions of turkey. methods: a hundred honey bee samples were collected from 99 apiaries in 11 different locations in 2011-2012 in turkey. nosema infection degree from collected sample...