cytotoxic effects of obturation materials were tested in presence and absence of endotoxin on human monocytes in vitro.human monocytes from thp-1 cell line were cultured. three millimeters from the tip of each resilon and gutta percha points were cut and directly placed at the bottom of the culture wells. cultured cells were exposed to gutta percha (groups g1 and g2) and resilon (r1 and r2). te...

objective: menstrual blood stromal stem cells (mbscs) share some phenotypic and functional similarities with mesenchymal stem cells (mscs). mscs are shown to inhibit either the function or generation of different immune cells, including dendritic cells (dcs). however, data regarding mbscs’ potential effects on immune system cells are elusive. here, we examine whether mbscs affect the generation...

platelet adherence surrounding leucocytes in a rosette formation or platelet satellitism is a rare phenomenon. this finding has been observed almost exclusively in ethylenediaminetetraacetic acid treated blood at room temperature. the mechanism underlying this phenomenon is not fully understood. in many reports of platelet satellitism platelets clump to polymorphonuclear neutrophils in healthy ...

controversial immunomodulatory properties of bee venom (bv) have provided an appropriate field for more investigation. the aim of present research was to verify the effects of honeybee venom on matrix metalloproteinase activity and interferon production as well as cell proliferation in monocyte and fibroblast cell lines. the monocyte and fibroblast cell lines (k562, ht-1080, wehi-164) were used...

objective(s)lupus nephritis (ln) is frequently associated with a poor long-term prognosis. renal biopsy is the diagnostic method of choice in this condition. urine biomarkers have been mentioned in the diagnosis of ln. the study,s purpose was to evaluate the performance of urinary monocyte chemoattractant protein 1(umcp-1) as a biomarker of renal involvement in systemic lupus erythematosus.mate...

background: dendritic cells (dc) induce tumor or pathogen-specific t cell responses in humans. several laboratories have developed culture systems, including maturation factors for human dc from peripheral blood monocytes. we comprehensively compared standard maturation stimulus, an autologous monocyte-conditioned medium (mcm), with heparin for their ability to promote uniformly mature dc that ...

background: the immunomagnetic separation technique is the basis of monocyte isolation and further generation of monocyte-derived dendritic cells. objective: to compare the efficiency of monocyte positive and negative separation, concentration of beads, and their impact on generated dendritic cells. methods: monocytes were obtained using monoclonal antibody-coated magnetic beads followed the fi...