evidences from many studies suggested a polymerase chain reaction (pcr) as a valuable method for diagnosing infectious disease in the transplant recipients. we used this method for detection of toxoplasma, gondii and human cytomegalovirus in blood specimens from patients after bone marrow or kidney transplantation. dna of both infectious agents were detected using two separate sets of nested pr...

BACKGROUND & OBJECTIVE Glycoprotein B (gB), involved in cell-to-cell transmission of human cytomegalovirus (HCMV), is a critical factor in tissue tropism and viral pathogenesis. The aim of the present study was to compare the efficiency of PCR-based RFLP and multiplex nested PCR for gB gene of HCMV to determine their genotype in clinical specimens from patients with HCMV. METHODS The PCR base...

Using Single Multiplex PCR Reaction to Identify Candida Species in Vaginal isolates: Comparison between Phenotypic and Molecular Methods

The multiplex polymerase chain reaction (PCR) technique was applied to detect the SARS-CoV (severe acute respiratory syndrome-associated coronavirus) specific target cDNA fragments in the present study. The target cDNA fragments of SARS-CoV were synthesized artificially according to the genome sequence of SARS-CoV in GenBank submitted by The Chinese University of Hong Kong, and were used as sim...

BACKGROUND Control of the global burden of tuberculosis is obstructed due to lack of simple, rapid and cost effective diagnostic techniques that can be used in resource poor-settings. To facilitate the early diagnosis of TB directly from clinical specimens, we have standardized and validated the use of nested multiplex PCR, targeting gene fragments IS6110, MTP40 and 32kD α-antigen encoding gene...

BACKGROUND In this study, we evaluated the analytical performance and clinical potential of a one-step multiplex real-time PCR assay for the simultaneous detection of 14 types of respiratory viruses using the AdvanSure RV real-time PCR Kit (LG Life Sciences, Korea). METHODS Three hundred and twenty clinical specimens were tested with the AdvanSure RV real-time PCR Kit and conventional multipl...

Simple and reliable conventional multiplex-polymerase chain reaction (multiplex-PCR) as well as real-time PCR approaches for the partial-length cytochrome b or cyt b gene of mitochondrial DNA (mtDNA) were compared to identify seven animal species for the authenticity of low non Halal (pig, dog, cat and donkey) and high value Halal (cattle, sheep and goat) meats. Either analysis indicated the su...

Wuchereria bancrofti, Dirofilaria immitis, and Dirofilaria repens are filarial nematodes transmitted by mosquitoes belonging to Culex, Aedes, and Anopheles genera. Screening by vector dissection is a tiresome technique. We aimed to screen filarial parasites in their vectors by single and multiplex PCR and evaluate the usefulness of multiplex PCR as a rapid xenomonitoring and simultaneous differ...

A multiplex PCR was developed for detection of hemolysin-producing Vibrio anguillarum using primers targeting five hemolysin genes (vah1, vah2, vah3, vah4 and vah5). This method was successful in amplifying reactions containing as little as 100 fg of genomic template DNA. The direct detection of V. anguillarum in clinical specimens by this multiplex PCR was also successful in reactions containi...

OBJECTIVES To evaluate the efficacy of Multiplex Ligation-dependent Probe Amplification (MLPA) technique in comparison with the traditional multiplex PCR assay in detection of exon deletions and duplications of the DMD gene. DESIGN AND METHODS The sensitivity and accuracy of MLPA were assessed and compared with the multiplex PCR in a total of 63 subjects including 43 subjects with Duchenne mu...