background and aims: certification programs of plant propagating materials rely on faster, cheaper and more importantly sensitive and reliable methods for detection of systemic pathogens as indicated in national and/or international health standards of plant propagating materials. reverse transcription-polymerase chain reaction (rt-pcr) has been documented as an alternative assay for certificat...

aim : we aimed to develop a multiplex pcr assay for specific detection of epec and ehec pathotypes based on specific marker genes. background : about 2.5 million infant’s morbidity in developing countries occurs by e.coli pathotypes because of diarrhea and intestinal diseases. the traditional phenotypic methods are time consuming and sometimes detection and differentiation of the pathotypes are...

background and objectives: dna ladder contains dna fragments of different length but with known size, used to determine the size of unknown dna molecules. different dna ladders are available for expected dna length. conserved sequences were selected for design of primers to generate dna fragments of known specific size. materials and methods: in this study, we describe a method by which dna lad...

background and objectives: we developed and evaluated the utility of a quadruplex taqman real-time pcr assay that allows simultaneous identification of vancomycin-resistant genotypes and clinically relevant enterococci. materials and methods: the specificity of the assay was tested using reference strains of vancomycin-resistant and susceptible enterococci. in total, 193 clinical isolates were ...

today, it is nearly 25 years past from investigation of shiga toxigenic escherichia coli (stec) which is able to produce shiga toxins and cause different gastroenteritis. since incidence of gastroenteritis due to stec is increasing, it's necessary to develop rapid, specific and accurate procedures like pcr. in this study, we used pcr method to detect and identify stec in cultures of 55 escheric...

objective: methicillin-resistant staphylococcus aureus is a major cause of nosocomial and community-acquired infections. aminoglycosides are potent bactericidal agents that are often used in combination with either a β-lactam or a glycopeptide, especially in the treatment of staphylococcal endocarditis. the main mechanism of aminoglycoside resistance in staphylococci is drug inactivation by cel...