We performed spoligotyping and 24-locus mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) typing on M. tuberculosis culture-positive biopsy specimens collected from adults dying in a hospital in KwaZulu-Natal. Of 56 culture-positive samples genotyped, we detected mixed strains in five (9%) and clonal heterogeneity in an additional four (7%).

Our patient was a 4-year-old female with acute myeloid leukemia complicated right calcaneal osteomyelitis due to Mycobacterium abscessus subcutaneous abscesses extending the popliteal and groin regions after two courses of induction chemotherapy according NOPHO-AML 2012 protocol. She required multiple operations prolonged anti-mycobacterial therapy. A high index suspicion for mycobacterial infe...

three tuberculous, twenty-one non-tuberculous mycobacterial (ntm) reference strains and seventy two isolates classified by biochemical tests were shown to produce specific sets of dna fragments in a polymerase chain reaction with single universal primer (up-pcr). a rather wide limit of tolerance for variations in procedure of pcr mixture preparation and thermocycling parameters was found. there...

background bcg (bacille calmette-guerin) vaccine is an attenuated live vaccine administered to prevent tuberculosis. disseminated infection due to bcg is a rare life threatening complication of this vaccine, especially in immunocompromised patients. objectives the current study evaluated the sensitivity pattern of the bcg strain supplied by the institute de pasteur, iran against anti-mycobacter...

identification of acid-fast bacilli (afb) in sputum or tissue samples is among definite diagnostic methods of tuberculosis. however, this method of diagnosis is restricted by certain limitations.   serologic diagnosis of tuberculosis (tb) has been used for a long time. the aim of this study was to determine the sensitivity and , specificity of antigen 60 (a60) igg, iga, igm test results in tb d...

Increasing evidence indicates that the risk of acquiring tuberculosis (TB) and nontuberculous mycobacterial disease is elevated among patients with rheumatoid arthritis (RA). To determine the epidemiology of mycobacterial diseases among RA patients in Asia, we conducted a retrospective cohort study. We used a nationwide database to investigate the association of RA with mycobacterial diseases. ...

In order to create a system in which two independent plasmids can be integrated into a mycobacterial chromosome, a mycobacterial plasmid was constructed containing the phage attachment site attP from the mycobacteriophage L5 genome and additionally containing the bacterial attachment site, attB. This plasmid will integrate into the mycobacterial chromosome via recombination of the plasmid-borne...

BACKGROUND Prosthetic joint infection (PJI) is a major complication of total hip and total knee arthroplasty (THA, TKA). Although mycobacteria are rarely the causative pathogens, it is important to recognize and treat them differently from non-mycobacterial infections. This study aimed to compare the clinical characteristics, associated factors and long-term outcomes of mycobacterial and non-my...

Tuberculosis remains one of the major threats to public health worldwide. Given the prevalence of multi drug resistance (MDR) in Mycobacterium tuberculosis strains, there is a strong need to develop new anti-mycobacterial drugs with modes of action distinct from classical antibiotics. Inhibitors of mycobacterial virulence might target new molecular processes and may represent a potential new th...

A multiplex method using the SNaPshot technique was developed to screen for six common mycobacterial species: Mycobacteria tuberculosis, M. avium, M. intracellulare, M. chelonae, M. kansasii, and M. gordonae. A total of 468 mycobacterial clinical isolates were subjected to analysis for the presence of the six mycobacterial species by the multiplex SNaPshot method. Of the 468 mycobacterial isola...