In plant mitochondria, gene expression of translatable mRNAs is a complex process with two critical steps, RNA editing and splicing. We studied the role of RNA editing on non-coding regions of the mat-r-nad1e-nad5c transcript from wheat mitochondria. This RNA contains two trans-introns, 3'-nad1-I4 and 3'-nad5-I2, involved in different trans-splicing events, ensuring the association of nad1d-nad...

Abstract Strawberry ( Fragaria × ananassa ) is native to temperate regions. However, it has been produced in tropical areas, as a seasonal crop including Florida, USA during the winter months. In March 2022, root galls resembling those induced by root-knot nematodes Meloidogyne spp.) were observed declining strawberry plants ‘Winterstar TM FL 05-107’ growing an organic-certified research site H...

The family Miridae is one of the most species-rich families of insects. To better understand the diversity and evolution of mirids, we determined the mitogenome of Lygus pratenszs and re-sequenced the mitogenomes of four mirids (i.e., Apolygus lucorum, Adelphocoris suturalis, Ade. fasciaticollis and Ade. lineolatus). We performed a comparative analysis for 15 mitogenomic sequences representing ...

The mitochondrial single-copy gene nad5 of wheat and maize consists of 5 exons located on three widely separated regions of the genome that are independently transcribed. The first region contains exons I and II separated by an atypical group II intron; in the second region is exon III (only 22 bp long), which is flanked upstream by a maturase-related open reading frame (ORF) and exon e of the ...

We report the complete mitochondrial genome sequence of Lichtheimia ramosa (syn. Lichtheimia hongkongensis), the first complete mitochondrial DNA sequence of the genus Lichtheimia. This 31.8-kb mitochondrial genome encodes 11 subunits of respiratory chain complexes, 3 ATP synthase subunits, 25 tRNAs, and small and large rRNAs, with the gene order atp9-cox2-atp6-cox3-cox1-nad2-nad3-cob-nad1-nad6...

This study involved 20 patients affected by cystic echinococcosis (CE) who were referred to different hospitals of Sardinia (Italy) from 2017 2022. By means a multidisciplinary approach, diagnosis was confirmed for CE in 18 and aetiologies two subjects. Moreover, serology positive 15 Since multiple cysts found five patients, total 27 lesions collected; however, only one each patient investigate...

background and aims: certification programs of plant propagating materials rely on faster, cheaper and more importantly sensitive and reliable methods for detection of systemic pathogens as indicated in national and/or international health standards of plant propagating materials. reverse transcription-polymerase chain reaction (rt-pcr) has been documented as an alternative assay for certificat...

RNA editing plays an important role in organelle gene expression in various organisms, including flowering plants, changing the nucleotide information at precise sites. Here, we present evidence that the maize (Zea mays) nuclear gene Pentatricopeptide repeat 2263 (PPR2263) encoding a DYW domain-containing PPR protein is required for RNA editing in the mitochondrial NADH dehydrogenase5 (nad5) an...

Human diphyllobothriasis is a widespread fish-borne zoonosis caused by the infection with broad tapeworms belonging to the genus Diphyllobothrium. In mainland China, so far 20 human cases of Diphyllobothrium infections have been reported, and the etiologic species were identified as D. latum and D. nihonkaiense based on morphological characteristics or molecular analysis. In the present study, ...

A long-term genetic legacy of refugial isolation has been postulated and was demonstrated for maternal refugial lineages for numerous plant and animal species. The lineages were assumed to have remained separated from each other for several glacial periods. The conifer Abies alba Miller, silver fir, is an excellent model to test whether pollen-mediated gene flow may eliminate the genetic imprin...