background: male are involved in near 50% of cases of infertility and reactive oxygen species (ros) playing an important role in decreasing fertility potential. accurate measurement of ros seems to be important in evaluation of infertile male patients. objective: to compare ros measurement in neat and washed semen samples of infertile men and define the best method for evaluation of ros in thes...

OBJECTIVE To investigate sperm viability, incidence of apoptosis, and intracellular basal and induced reactive oxygen species (ROS) in sperm fractions. DESIGN Prospective controlled study. SETTING Center for Reproductive Medicine at a tertiary care hospital. METHOD(S) Liquefied seminal ejaculates (n = 12) prepared by density gradient centrifugation were reconstituted to 2 mL with phosphat...

PURPOSE Our aim was to evaluate the effect of neat semen vitrification on human sperm vital parameters and DNA integrity in men with normal and abnormal sperm parameters. MATERIALS AND METHODS Semen samples were 17 normozoospermic samples and 17 specimens with abnormal sperm parameters. Semen analysis was performed according to World Health Organization (WHO) criteria. Then, the smear was pro...

AIM To compare the seminal attributes of neat, pre-freeze (at equilibration), and post-freeze (24 h after freezing) semen in pure and crossbred Jersey bulls. MATERIALS AND METHODS Total 36 ejaculates (3 ejaculates from each bull) were collected from 6 pure Jersey and 6 crossbred Jersey bulls and evaluated for various seminal attributes during neat, pre-freeze, and post-freeze semen. RESULTS...

OBJECTIVE To examine simultaneously the levels of hydrogen peroxide (H(2)O(2)) and superoxide (O(2)(-*)) using chemiluminescence and flow cytometry. DESIGN Prospective laboratory study. SETTING Reproductive research lab in a tertiary hospital. PATIENT(S) Semen samples from 18 healthy male volunteers. INTERVENTION(S) Sperm preparation and measurement of reactive oxygen species (ROS) by c...

The predictive value of sperm chromatin integrity for pregnancy outcome following in-vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) was studied in 24 men attending a university-based assisted reproductive techniques laboratory using the flow cytometric sperm chromatin structure assay (SCSA). The SCSA is a measure of the susceptibility of sperm DNA to low pH-induced denatu...

BACKGROUND Detection of apoptosis in sperm samples may help evaluate sperm quality. Recently, it has been suggested that in some ejaculated sperm populations, apoptosis is caspase dependent. The aim of this study was to investigate the presence of activated caspases and examine possible correlations with apoptosis and sperm parameters in semen samples prepared for IVF. METHODS To detect activ...

BACKGROUND Sperm cell death appears to be a cause of male infertility. The objective of this study was to determine the most reliable method for the evaluation of sperm quality in semen samples during sperm preparation for IVF. METHODS Conventional analysis of semen samples was compared with several cytofluorometric methods detecting death-associated changes. Neat semen from infertile patient...

OBJECTIVE To evaluate effects of cellular phone radiofrequency electromagnetic waves (RF-EMW) during talk mode on unprocessed (neat) ejaculated human semen. DESIGN Prospective pilot study. SETTING Center for reproductive medicine laboratory in tertiary hospital setting. SAMPLES Neat semen samples from normal healthy donors (n = 23) and infertile patients (n = 9). INTERVENTION(S) After l...

BACKGROUND The swim-up and hyaluronan (HA)-binding methods are used for the selection of good quality spermatozoa to improve pregnancy rates and embryo quality and to reduce the number of miscarriages after IVF. We evaluated whether the processing of sperm by these methods reduces the frequency of spermatozoa with abnormal karyotypes and altered chromatin quality in balanced translocation carri...