This opinion reports on an evaluation of a risk assessment for placing on the market the genetically modified herbicide tolerant and insect resistant maize MON89034 x NK603 for food and feed uses, import and processing. Conventional breeding methods were used in the production of maize MON89034 x NK603 from inbred lines of the respective parental events. The structural integrity of the inserts ...

In this study, the event-specific primers for insecticide-resistant maize, MON810, and herbicide-tolerance maize, NK603, have been designed. Simplex PCR and multiplex PCR detection method have been developed. The detection limit of the multiplex PCR is 0.5% for MON810 and NK603 in 50 ng of the template for one reaction. Quantitative methods based on real-time quantitative PCR were developed for...

Glyphosate tolerant genetically modified (GM) maize NK603 was assessed as 'substantially equivalent' to its isogenic counterpart by a nutrient composition analysis in order to be granted market approval. We have applied contemporary in depth molecular profiling methods of NK603 maize kernels (sprayed or unsprayed with Roundup) and the isogenic corn to reassess its substantial equivalence status...

Monitoring of genetically modified (GM) crops has been emphasized to prevent their potential effects on the environment and human health. Monitoring of the inadvertent dispersal of transgenic maize in several fields and transport routes in Korea was carried out by qualitative multiplex PCR, and molecular analyses were conducted to identify the events of the collected GM maize. Cytogenetic inves...

Our recent work (Séralini et al., 2012) remains to date the most detailed study involving the life-long consumption of an agricultural genetically modified organism (GMO). This is true especially for NK603 maize for which only a 90-day test for commercial release was previously conducted using the same rat strain (Hammond et al., 2004). It is also the first long term detailed research on mammal...

genetically modified (gm) foods hold the key to ending hunger and malnutrition in africa.   due to the increasing number of gmos cultivation and delay in the approval of biosafety law in nigeria, it has become necessary to screen maize products in order to determine the identity of the consumed daily foodstuffs. in this study, dna extraction from raw and processed maize foods sold commercially ...

Maize 1507 × MIR162 MON810 NK603 (four-event stack maize) was produced by conventional crossing to combine four single events: 1507, MIR162, and NK603. The GMO Panel previously assessed the events six of subcombinations did not identify safety concerns. No new data on or that could lead modification original conclusions their were identified. molecular characterisation, comparative analysis (ag...

BACKGROUND A previous 2-year rat feeding trial assessing potential toxicity of NK603 Roundup-tolerant genetically modified maize revealed blood and urine biochemical changes indicative of liver and kidney pathology. In an effort to obtain deeper insight into these findings, molecular profiling of the liver and kidneys from the same animals was undertaken. RESULTS Transcriptomics showed no seg...

A comparison was made of the effect of glyphosate (Roundup®Plus), a post-emergency applied herbicide, and of Harness®GTZ, a pre-emergency applied herbicide, on the rhizobacterial communities of genetically modified NK603 glyphosate-tolerant maize. The potential effect was monitored by direct amplification, cloning and sequencing of soil DNA encoding 16S rRNA, rhizobacterial DNA hybridization to...

http://www.efsa.eu.int 1 Opinion of the Scientific Panel on Genetically Modified Organisms on a request from the Commission related to the safety of foods and food ingredients derived from herbicide-tolerant genetically modified maize NK603, for which a request for placing on the market was submitted under Article 4 of the Novel Food Regulation (EC) No 258/97 by Monsanto1 (QUESTION NO EFSA-Q-20...