glioblastoma is the most common and the most lethal primary brain cancer. this malignancy is highly locally invasive, rarely metastatic and resistant to current therapies. little is known about the distinct molecular biology of glioblastoma multiforme (gbm) in terms of initiation and progression. so far, several molecular mechanisms have been suggested to implicate in gbm development. homeodoma...

background: the canonical wnt signal transduction (or the wnt/β-catenin pathway) plays a crucial role in the development of animals and in carcinogenesis. beta-catenin is the central component of this signaling pathway. the activation of wnt/β-catenin signaling results in the cytoplasmic and nuclear accumulation of β-catenin. in the nucleus, β-catenin interacts with the tcf/lef transcription fa...

the association of secreted frizzled related protein type 4 (sfrp4) as an antagonist of wnt mole-cules in apoptotic events has been reported previously. moreover, its increased expression has been reported in the ovary of women with polycystic ovary (pco). we have demonstrated in-creased sfrp4 in pco-induced rat ovary related to an increased number of apoptotic follicles showing nuclear ?cateni...

The association of secreted frizzled related protein type 4 (Sfrp4) as an antagonist of Wnt mole-cules in apoptotic events has been reported previously. Moreover, its increased expression has been reported in the ovary of women with polycystic ovary (PCO). We have demonstrated in-creased Sfrp4 in PCO-induced rat ovary related to an increased number of apoptotic follicles showing nuclear ?cateni...

agrobacterium tumefaciens is capable of gene transfer to both plant and non-plant organisms. indeed, upon infection of eukaryotic cells, agrobacterium tumefaciens transfers a piece of its tumor inducing (ti)-plasmid, called t-dna, to the host cell nucleus, which subsequently integrates into the host genome. the vird2 virulence protein which has relaxase endonuclease activities covalently binds ...

Background: Involvement of Wnt proteins and one of its antagonist known as secreted Frizzled Related Protein type-4 (sFPRP-4) was reported in rodent ovarian follicular development. Other studies showed an ap- Abstracts of the 11th Royan International Congress on Reproductive Biomedicine 7 7 International Journal of Fertility & Sterility (IJFS), Vol 4, Suppl 1, Summer 2010 optotic-associated exp...

background: the aim of this study was to clone pparγ1 cdna in an appropriate mammalian expression vector, with a chimeric cdna form, encompassing pparγ with enhanced green fluorescent protein (egfp) cdna. this recombinant plasmid will be used for further analyses to investigate the molecular mechanism of pparγ1 for neural differentiation process. moreover, the nuclear localization of the pparγ1...

Of the >40 alternative and aberrant splice variants of MDM2 that have been described to date, the majority has lost both the well-characterized nuclear localization signal (NLS1) and the nuclear export signal (NES) sequence. Because cellular localization of proteins provides insight regarding their potential function, we determined the localization of three different MDM2 splice variants. The s...

Approximately 100 proteins are targeted to the inner nuclear membrane (INM), where they regulate chromatin and nuclear dynamics. The mechanisms underlying trafficking to the INM are poorly understood. The Caenorhabditis elegans SUN protein UNC-84 is an excellent model to investigate such mechanisms. UNC-84 recruits KASH proteins to the outer nuclear membrane to bridge the nuclear envelope (NE),...