Polyspermy or the penetration of more than one sperm cell remains a problem during porcine in vitro fertilization (IVF). After in vitro culture of porcine zygotes, only a low percentage of blastocysts develop and their quality is inferior to that of in vivo derived blastocysts. It is unknown whether the cytoplasmic maturation of the oocyte is sufficiently sustained in current in vitro maturatio...

Controversy exists about the risk of microbiological contamination from direct contact with unsterile liquid nitrogen during oocyte vitrification. The aim of this observational study was to evaluate the effectiveness of oocyte vitrification using a high-security closed vitrification system in a donation programme. Oocyte vitrification was performed using CBS High Security closed straws (Cryo Bi...

BACKGROUND The enzyme hyaluronidase from bovine origin is commonly used for oocyte-cumulus cell removal in ICSI. A recombinant human hyaluronidase (rHuPH20) has been introduced as a quality-controlled and safe alternative. METHODS In order to validate its effectiveness, a non-inferiority trial was started on sibling cumulus-oocyte complexes (135 ICSI patients). Oocyte denudation involved enzy...

background: determination of oocyte and embryo quality are one of the most important goals in ivf. anti-mullerian hormone (amh) is secreted by the ovarian granulosa cells into blood flow and follicular fluid. follicular fluid anti-mullerian hormone level is probably a marker of activity of granulose cells. objective: to evaluate whether high level of follicular fluid anti-mullerian hormone leve...

It is well known that maternal ageing not only causes increased spontaneous abortion and reduced fertility, but it is also a high genetic disease risk. Although assisted reproductive technologies (ARTs) have been widely used to treat infertility, the overall success is still low. The main reasons for age-related changes include reduced follicle number, compromised oocyte quality especially aneu...

The aim of this study was to determine the quality of cumulus-oocyte-complexes (COCs) and the effects of bovine serum albumin (BSA) supplementation on in vitro maturation and fertilization rate of buffalo oocytes. COCs were collected from slaughterhouse buffalo ovaries by aspiration method. Only normal grades COCs were matured for 48 hours in TCM-199 media. Two groups were created: one for the ...

The zygote of sexually reproducing organisms contains a combination of parental genomes, and all subsequent cells of the embryo are derived from this original genotype. Although clonal, it is not known howmuch genetic variation exists in progeny of this original cell, or between cells of the same lineage resulting from this zygote. Oocytes in mammals, especially humans, have prolonged developme...

Recently, studies on inositol supplementation during in vitro fertilization program (IVF) have gained particular importance due to the effect of this molecule on reducing insulin resistance improving ovarian function, oocyte quality, and embryo and pregnancy rates and reducing gonadotropin amount during stimulation. Inositol and its isoforms, especially myoinositol (MYO), are often used as pres...

BACKGROUND Fat-rich diet may alter oocyte development and maturation and embryonic development by inducing oxidative stress (OS) in follicular environment. OBJECTIVE To investigate the relationship between fat intake and oxidative stress with oocyte competence and embryo quality. MATERIALS AND METHODS In observational study follicular fluid was collected from 236 women undergoing assisted r...