Organophosphorus hydrolase can effectively degrade organic phosphorus compounds such as sarin. In this study, we constructed a recombinant Bacillus subtilis mutant expressing organophosphorus hydrolase, measured the effect of on degradation rate nerve agent sarin, and selected optimal mutation scheme. Three different genes, 257L, 257Y 303T, were ligated to PMA0911 vector transferred into WB800 ...

The promising results obtained with certain organic phosphorus compounds such as Dow-ET-14 and Dow-ET-57 against Hypoderma sp. (McGregor, Radeleff & Bushland, 1954; McGregor & Bushland, 1957; Adkins, 1957; Roth & Gaines, 1957), indicated the possible use of these systemic insecticides for the treatment of specific oculo-vascular myiasis (Basson, 1962). The heavy losses sustained by the farmers ...

Most organophosphorus esters are direct inhibitors or are rapidly converted to inhibitors of acetylcholinesterase (AChE, EC 3.1.1.7) (1-3). Some of these compounds produce a more persistent effect: delayed neurotoxicity. In humans, organophosphorus ester-induced delayed neurotoxicity (OPIDN) results in a flaccid paresis which develops distally in the legs and spreads to the hands and thighs. In...

A novel capacitive field-effect sensor for the direct determination of organophosphorus pesticides by the enzyme organophosphorus hydrolase (OPH) is presented. The enzyme OPH hydrolyses organophosphorus compounds catalytically, thus releasing Hþ ions. By means of a silicon-based field-effect sensor, which consists of a layer sequence of Al/p-Si/SiO2 with alternative pH-sensitive materials (Ta2O...

In the present scenario, usage of pesticides are inevitable to meet the demands of growing population and to secure the food grain production, there is need for regular screening of milk and milk products for pesticide residues which is being felt in the trade and also at the consumers level. The present work was undertaken to study the effect of heat processing on degradation of various Organo...

Organophosphorus chemistry has a crucial role in the field of drug research [1]. As generally well known, Adenosine Triphosphate (ATP) plays key energy processes all living cells mammals. There are also natural phosphorus containing organic compound, Phosphocitrate (PC), found mammalian mitochondria, likely produced by cytosolic phosphorylation citrate, and which important calcium metabolism, e...

Organophosphorus compounds are ubiquitous in nature, and due to their innate chemical properties, serve a fundamental role in a number of important fields. Among the more prominent features that elevate their status as a unique and versatile class of compounds, include variable oxidation states, multivalency, asymmetry and metal-binding properties. Their presence in bioactive natural products, ...