Vascular endothelial growth factor A (Vegfa) has important roles in endochondral bone formation. Osteoblast precursors, endothelial cells and osteoclasts migrate from perichondrium into primary ossification centers of cartilage templates of future bones in response to Vegfa secreted by (pre)hypertrophic chondrocytes. Perichondrial osteolineage cells also produce Vegfa, but its function is not w...

The synthesis of type I collagen, the main component of bone matrix, precedes the expression of Runx2, the earliest determinant of osteoblast differentiation. We hypothesized that the energetic needs of osteoblasts might explain this apparent paradox. We show here that glucose, the main nutrient of osteoblasts, is transported in these cells through Glut1, whose expression precedes that of Runx2...

Titanium has an osseointegrative property, while hydroxyapatite has an osteoconductive property. It remains a matter of controversy among researchers whether hydroxyapatite has higher osteoblast compatibility than titanium. Here, we compared the activities between osteoblasts cultured on titanium and those cultured on hydroxyapatite. An osteoblast-like cell line, MC3T3-E1, was cultured on machi...

Graphdiyne-modified TiO 2 nanofibers, which could be incorporated into orthopedic implants, have impressive self-antibacterial and osteoinductive abilities due to their efficient ROS production, favorable osteoblast adsorption, good biocompatibility.

BACKGROUND: Stem cells have great effects in clinical cell-based therapy. Accordingly, controlling the behavior and directing the fate of stem cells cultured in the laboratory is an important issue. OBJECTIVES: The aim of this study was to evaluate osteogenic properties of adipose derived mesenchymal stem cells (ADSCs) which differentiated toward osteogenic linage by osteoblast-imprinted substr...

Vertically aligned yet laterally spaced nanoscale TiO2 nanotubes have been grown on Ti by anodization, and the growth of MC3T3-E1 osteoblast cells on such nanotubes has been investigated. The adhesion/propagation of the osteoblast is substantially improved by the topography of the TiO2 nanotubes with the filopodia of growing cells actually going into the nanotube pores, producing an interlocked...

Approved: ______________________________________________ Thesis Supervisor ______________________________________________ Title and Department ______________________________________________ Date THE ISOPRENOID BIOSYNTHESIS PATHWAY AND REGULATION OF OSTEOBLAST DIFFERENTIATION by Megan Moore Weivoda A thesis submitted in partial fulfillment of the requirements for the Doctor of Philosophy degree ...

Previous studies have demonstrated increased functions of osteoblasts (bone-forming cells) on nanophase compared to conventional ceramics (specifically, alumina, titania, and hydroxyapatite), polymers (such as poly lactic-glycolic acid and polyurethane), carbon nanofibers/nanotubes, and composites thereof. Nanophase materials are unique materials that simulate dimensions of constituent componen...

We have studied the survival requirements of osteoblasts to test the hypothesis that osteoblasts undergo programmed cell death (PCD) or apoptosis unless they are continuously signalled by other cells not to do so. Osteoblasts survived for 6 days in culture at high cell density in the absence of other cell types, serum or exogenous proteins, but they died with the morphological features of apopt...

The extracellular matrix glycoprotein, tenascin, is associated in vivo with mesenchyme undergoing osteogenesis and chondrogenesis, but is absent from mature bone and cartilage matrix. The expression of tenascin by osteoblastic cells in vitro has been investigated by immunoblotting and immunocytochemistry. Tenascin was secreted into the medium and deposited in the matrix by human and rat osteobl...