one which is now widely used for diagnostic purposes. The difficulties involved in making a permanent accurate record of the results, an essential part of the technique, are indicated by the variety of photographic methods that have been used, the majority of which are time-consuming and involve the use of special photographic equipment. We have been using a modification of a method first descr...

Cryoprecipitation was observed in defibrinated serum from Schistosoma japonicum-infected rabbits. Ouchterlony immunodiffusion, sodium dodecyl sulfate-polyacrylamide gels, and anti-cryoprecipitate antiserum demonstrated the presence of immunoglobulin G (IgG), IgM, C3, fibrinogen, and an alpha-macroglobulin. Parasite antigen was not detected. IgG and IgM from pooled cryoprecipitates failed to rea...

Antisera against whole cells of each Peptostreptococcus species (P. anaerobius, P. micros, P. parvulus, and P. productus) were produced in rabbits. When these antisera were reacted against sonically disrupted cells and culture supernatant fluids in Ouchterlony tests, lines of identity were obtained among the antigens from all the species and uninoculated culture medium. When the antisera were s...

Thirty-two antisera against the hepatitis-associated antigen (HAA) were studied for titer and specificity. Titers were expressed in milligrams of the antigen precipitated by one milliliter of the antiserum. Specificity was investigated by Ouchterlony double diffusion against 40 HAA-positive sera and a serum pool consisting of about 200 HAA-positive specimens. Antiserum titers ranged from 0.04 m...

We investigate pattern avoidance in alternating permutations and an alternating analogue of Young diagrams. In particular, using an extension of Babson and West’s notion of shape-Wilf equivalence described in our recent paper (with N. Gowravaram), we generalize results of Backelin, West, and Xin and Ouchterlony to alternating permutations. Unlike Ouchterlony and Bóna’s bijections, our bijection...

Serum transaminases were determined according to the method ofKarmen, Wrobleski, and LaDue (1955) (normal values for our laboratory: SGOT and SGPT up to 15 i.u./ litre), serum salicylate levels with Trinder's reagent, fibrinogen according to the method of Clauss (1957), fibrinogen degradation products (FDP) by the immunodiffusion technique (Ouchterlony), and Factor V according to the method of ...

background: equine chorionic gonadotropin (ecg) is commonly used in association with a progestagen treatment to synchronize estrus of goats and ewes during breeding and non-breeding seasons. classical purification of the ecg from serum includes ph fractionation with metaphosphoric acid two ethanol precipitation steps as well as dialysis followed by fixed-bed chromatography. objectives: the aim ...

Pancreatic juice collected from 10 patients without evidence of malignant disease of the pancreas or other organs was pooled, extracted, and fractionated by Sepharose 6-B and Sephadex G-200 gel filtration. The carcinoembryonic antigen (CEA) activity in the material was demonstrated and studied by: a) radioimmunoassay, b) competitive binding to antibodies against CEA, c) precipitin inhibition, a...