The polymerase chain reaction (PCR) has made possible the rapid isolation and amplification of specific DNA segments, which can then be used for a wide range of applications (1). Direct sequencing of PCR products has been described, using assymetric PCR, which requires purification of the PCR product, or inclusion of 10% DMSO in the sequencing reaction (2, 3, 4). However, these methods of seque...

Merkel cell polyomavirus (MCPyV) is the etiological agent of majority carcinoma (MCC): a rare skin tumor. To improve our understanding role MCPyV in MCCs, detection and analysis DNA transcripts were performed on primary tumors regional lymph nodes from two MCC patients: one metastatic non-metastatic. MCPyV-DNA was searched by quantitative polymerase chain reaction (qPCR), followed amplification...

Either PCR-mediated single strand conformation polymorphism (SSCP) analysis or DNA sequencing of rpoB DNA (157 bp) can be used as a rapid screening method for the detection of mutations related to the rifampin resistance of Mycobacterium tuberculosis. However, due to the nonspecific amplification of rpoB DNA from nontuberculous mycobacteria these methods cannot be directly applied to clinical s...

We describe a method for direct cycle sequencing of PCR fragments amplified from genomic DNA or cDNA. DNA sequencing template is amplified using PCR and oligonucleotide primers flanking the region of interest. The amplified fragment is directly cycle sequenced using fluorescent sequencing primers, Sanger dideoxy sequencing chemistry and an enzyme mixture of a mutant Taq DNA polymerase and therm...

Background:  With one-third of the world’s population infected, tuberculosis (TB) is one of the most common infectious diseases and a major public health problem, especially in developing countries. The efficacy of the BCG vaccine for controlling the disease in adults is poor. The development of an effective TB vaccine is a global objective. An effective tuberculosis vaccine should s...

The application of bacteriophages biocontrol requires the formulation genetically distinct in a phage cocktail. Random Amplified Polymorphic DNA (RAPD) - PCR is considered cheap, reproducible, and readily applicable tool detecting diversity compared to other molecular techniques such as whole-genome sequencing. We used this study RAPD-PCR technique assess genetic 28 infecting Pseudomonas aerugi...