The thermal resistance of Clostridium sporogenes PA 3679 ATCC 7955 was determined in soymilk (pH 7) and 0.1% peptone water (pH 7) by the capillary tube method. In the continuous flow high-pressure throttling, the temperature of soymilk increased due to instantaneous pressure release and the additional heat was supplied by a heat exchanger to achieve the set temperature. The soymilk was immediat...

To examine the adaptation of bacterioplankton communities in the Southern Ocean to various biopolymeric substrates, we carried out experiments at the Polar Front and in the Antarctic Coastal Current (CC) in which samples were enriched with agarose, starch, peptone and extracts of the green alga Scenedesmus acutus and diatoms from the Polar Front. Growth and metabolic activity were assessed base...

background: recombinant monoclonal antibodies have been marketed in last three decades as the major therapeutic proteins against different cancers. however choosing a proper medium and supplements to reach the high expression is a challenging step. despite of commercial serum free and chemically defined media, there are still numerous researches seeking the optimum media to gain higher expressi...

The optimization of cell culture conditions for growth and productivity of recombinant Chinese hamster ovary (CHO) cells is a critical step in biopharmaceutical manufacturing. In the present study, the effects of the timing and amount of peptone feeding of a recombinant CHO cell line grown in a basal medium in serum-free suspension culture were determined for eight peptones of different origin ...

Investigation various nitrogen sources effects on the production of invertase by Aspergillus niger was researched in this study. Invertase is a precious enzyme used many industries like food, pharmacy, confectionery, invert syrup production. Taguchi design experiment (DOE) preferred to optimize cultivation conditions. L16 (43) orthogonal array selected current study including source, initial pH...

We studied nine solid and two liquid media for their suitability to select Aeromonas and Plesiomonas spp. from human stools, using artificially contaminated samples as well as 254 samples from outpatients with and without diarrhea. Media with optimal sensitivity and specificity for Aeromonas spp. were alkaline peptone-water, Trypticase soy broth with ampicillin, inositol-brilliant green-bile sa...