Polarographie reduction of adenine and cytosine moieties in denatured DNA was followed by means of differential (derivative) pulse polarography. The measurements of the reduction currents yielded by samples of denatured DNA differing in guanine+cytosine (G + C) content showed that both cytosine and adenine residues were reduced in DNA in the pH range of 6.0 —8.7. The con­ tribution of their red...

Essential to the renewal of interest in polarography and voltammetry in the 1970s were the availability of reliable polarographs offering the pulse mode, with sensitivity down to lo-‘-lo-* M and h’gh 1 er resolving power; a better understanding of the electrode processes of compounds of pharmaceutical interest (active agents, excipients, additives, antioxidants, etc.); and the application of ox...

Dionýz Ilkovič (Figure 1) was a Czechoslovak physicist and physical chemist. He made fundamental contributions to the theoretical background of polarography electroanalytical chemistry in general. Polarography is first technique that performs voltammetric study with mercury-dropping electrode 2A). This invented 1922 by Czech chemist Jaroslav Heyrovský, who received Nobel prize 1959 for inventio...

Optimum conditions for the determination of lanthanum at sub μg level have been developed employing differential pulse polarography. A detection limit of 0.5μg/ml has been achieved. The method successfully been applied for analysis of lanthanum in industrial wastes.

Transition of single-stranded poly(A) into its double-helical protonated form was followed by means of derivative pulse polarography, spectrophotometry, and other methods. It was found that properties of protonated poly(A) depended on the length of single strands from which the protonated double helix was formed. In contrary to longer poly(A) transition of short single-stranded molecules (s(20)...

Differential pulse polarography study was used to investigate the influence of glutathion on cis- and trans-DDP induced alterations of DNA structure. Though the applied concentration of glutathion has no effect on the reaction on DNA with cis-DDP, it greatly modifies the reaction with trans isomer. This may be an important reason for the ineffectiveness of the trans-DDP as an antitumor drug.

TYPES OF MELTS From the practical viewpoint, melts may be classified according to melting points which may limit the available techniques. The lowest melting, with working temperatures up to 250°, are typified by the LiNO3—NaNO3— KNO3 eutectic, NaNO3—KNO3 eutectic, LiClO4, and ammonium formate. With these solvents it is possible to use the dropping mercury electrode, or hanging mercury drop ele...

A method for determining citrate as its complex with iron(II1) by differential pulse polarography is described. The method is sensitive to 5 pg ml-1 of citrate, without any interference from equimolar propan-2-onedicarboxylate and propenetricarboxylate ions and 10% of oxalate and tartrate ions. The limits of error in citrate concentrations determined by the proposed method are better than &2.6y...

This is a review of analytical methods, such as spectrophotometry, derivative spectrophotometry and various chromatographic (gas chromatography-GC, high-performance liquid chromatography-HPLC, thin-layer chromatography-TLC, high-performance thin-layer chromatography-HPTLC, liquid chromatography-tandem mass spectrometry-LC-MS, microchip electrophoresis-MCE, capillary electrophoresis-CE) and elec...