Low seed yield is a limiting factor for cross breeding and hybrid seed production in cyclamen. This study was performed to investigate pollen germination and its relation to cross-pollination success and fruit set in this plant. In order to achieve a high level of pollen germination, the effect of different concentrations of chemical compounds were examined on in vitro pollen germination of cyc...

Pollen viability and its germination capability for fruit set in are almond and peach essential. To optimize the pollen culture medium of almond and peach and selection of best medium, the present study was carried out with 48 different culture media containing different compositions including diverse levels of boric acid (0 and 100mg/1), calcium nitrate (0,150and300mg/l،), magnesium sulphate (...

The relationship between pollen germination and the dynamic organization of the actin cytoskeleton during pollen germination is a central theme in plant reproductive biology research. Maize (Zea mays) pollen grains were implanted with 30 keV argon ion (Ar(+)) beams at doses ranging from 0.78 x 10(15) to 13 x 10(15) ions/cm(2). The effects of low-energy ion implantation on pollen germination via...

UNLABELLED BACKGROUND AND AIMS High-temperature environments with >30 degrees C during flowering reduce boll retention and yield in cotton. Therefore, identification of cotton cultivars with high-temperature tolerance would be beneficial in both current and future climates. * METHODS Response to temperature (10-45 degrees C at 5 degrees C intervals) of pollen germination and pollen tube gr...

Despite much effort, a robust protocol for in vitro germination of Arabidopsis thaliana pollen has been elusive. Here we show that controlled temperatures, a largely disregarded factor in previous studies, and a simple optimized medium, solidified or liquid, yielded pollen germination rates above 80% and pollen tube lengths of hundreds of microns, with both Columbia and Landsberg erecta (Ler) e...

There is minimal information about the effect of heavy metals such as lead and copper on pollen grains and pollen tubes of fruit trees. Fruit set of these plants are affected by different environmental, biological, physical and chemical factors. If one of these factors be abnormal, pollination, fertilization and fruit set and orchard yield will decrease. In polluted cities, this phenomenon is a...

Despite much effort, a robust protocol for in vitro germination of Arabidopsis thaliana pollen has been elusive. Here we show that controlled temperatures, a largely disregarded factor in previous studies, and a simple optimized medium, solidified or liquid, yielded pollen germination rates above 80% and pollen tube lengths of hundreds of microns, with both Columbia and Landsberg erecta (Ler) e...

Pollen quality is important for growers and breeders. This study was carried out to determine in vitro pollen viability and pollen germination in seven genotypes of cherry laurel (Prunus laurocerasus L.). Two pollen viability tests, TTC (2,3,5-triphenyl tetrazolium chloride) and IKI (iodine potassium iodide), were used. Pollen traits of genotypes were studied using an in vitro medium containing...

BACKGROUND AND AIMS The pollination to fertilization process (progamic phase) is thought to have become greatly abbreviated with the origin of flowering plants. In order to understand what developmental mechanisms enabled the speeding of fertilization, comparative data are needed from across the group, especially from early-divergent lineages. I studied the pollen germination process of Austrob...

The actin cytoskeleton is absolutely required for pollen germination and tube growth, but little is known about the regulation of actin polymer concentrations or dynamics in pollen. Here, we report that latrunculin B (LATB), a potent inhibitor of actin polymerization, had effects on pollen that were distinct from those of cytochalasin D. The equilibrium dissociation constant measured for LATB b...