The cell wall of 24-h zygotes of Fucus serratus is composed of 3 layers--an inner fibrillar layer (sulphated fucan), an outer fibrillar layer (alginic aicd/cellulose) and an exterior amorphous layer (sulphated fucan, alginic acid). The 2 layers containing sulphated fucan are preferentially thickened at the rhizoid pole. Light- and electron-microscope autoradiographic pulse-chase experiments on ...

A water-insoluble chitin-glucan complex, isolated from the mycelium of Aspergillus niger, was swollen in various aqueous media and treated subsequently by high-energy sonication. The concentration of the resulting water-soluble polysaccharide fractions was dependent on the swelling medium, the amount of the chitin-glucan complex in the suspension, and on the time of sonication. The yields of wa...

Box-Behnken design (BBD), one of the most common response surface methodology (RSM) methods, was used to optimize the experimental conditions for ultrasound-assisted extraction of polysaccharides from Rhynchosia minima root (PRM). The antioxidant abilities and anticancer activity of purified polysaccharide fractions were also measured. The results showed that optimal extraction parameters were ...

Undaria pinnatifida is a species of brown seaweed known to contain rich amounts of fucoidan, a sulfated polysaccharide known to possess various biological activities. We isolated crude fucoidan (F0) from the sporophylls of U. pinnatifida grown in the Marlborough Sounds, New Zealand. Sulfate content, uronic acid content, and molecular weight of F0 were 15.02, 1.24, and >150 kDa, respectively. F0...

The polysaccharide capsule which surrounds bacterial species like Haemophilus influenzae, Streptococcus pneumoniae, Neisseria meningitidis, Salmonella typhi, is a potent virulence factor. It protects the bacterium from phagocytosis, but capsule specific antibodies plus complement binding to the capsule opsonise the organism for phagocytosis and elimination. Purified capsules elicit T-independen...

Preparations of nucleic acids obtained by extraction of mouse liver, HeLa cells and cell fractions with phenol and deoxycholate have been characterized with regard to the differential solubility of ribonucleic acid and deoxyribonucleic acid in ethanol, density-gradient centrifugation and the presence of high-molecular-weight contaminants. Ribonucleic acid obtained by this method is less soluble...

When certain polysaccharide fractions isolated from cultures of Serra@tia marcescens (6, 2), Aero bader aerogenes, or other Gram-negative bacteria (8) are injected in large doses into mice, symptoms very similar to those of shock occur. The animals are prostrated, their muscular response is much lower than normal, they breathe with difficulty, and the body temperature drops. Similar symp toms a...

In the present study, properties of silver nanoparticles (AgNPs) such as average size, size distribution andmorphology were investigated by Tollens, polysaccharide, modified polysaccharide and microbial methods.The synthesized AgNPs were characterized by UV-visible spectroscopy, scanning electron microscopy(SEM), transmission electron microscopy (TEM), dynamics light scatterin...

This study describes the kinetics of (35)S-incorporation during in vivo sulfate esterification of Porphyridium aerugineum capsular polysaccharide. Techniques were developed to isolate the precursor pool (free sulfate), cell-associated product, and extracellular product. Specific radioactivities of these three fractions were monitored during pulse-chase sequences. Label rapidly appeared in the p...