An experiment was conducted for a period of eight weeks under laboratory conditions to determine the effect of different salinity levels on the survival and growth of crablings of mud crab, Scylla serrata. The different salinity levels tested were: 5 ppt, 10 ppt, 15 ppt, 20 ppt and 25 ppt. The differences in growth increment in terms of body weight (BW) were significant (p<0.05) among different...

In euryhaline crustaceans, sensitivity to toxic trace metals may be linked to osmoregulation and salinity conditions. This study investigated if grass shrimp (Palaemonetes pugio) populations from different salinity regimes differed in sensitivity to cadmium (Cd). Grass shrimp were collected in May 2011 from two marsh sites with average salinities of ~3.0 ppt and 24.0 ppt. Groups were acclimated...

Salinity tolerance to Thai silver barb, Barbodes gonionotus was investigated under laboratory conditions using common salt (NaCl) and sea water. With a view to determine the median lethal concentrations (LC50 values), the fish were exposed to various concentrations of the salt and sea water for a period of 96. Common salt up to a concentration of 12 ppt and sea water up to a concentration of 10...

Previous results using a SELEX (Systematic Evolution of Ligands by Exponential Enrichment)-based approach that selected DNA primer-template duplexes binding with high affinity to HIV reverse transcriptase (RT) showed that primers mimicking the 3' end, and in particular the six nt terminal G tract, of the RNA polypurine tract (PPT; HIV PPT: 5'-AAAAGAAAAGGGGGG-3') were preferentially selected. In...

PURPOSE To evaluate whether the pressure phosphene tonometer (PPT) is suitable for self-tonometry in patients with glaucoma or ocular hypertension. METHODS This was a prospective comparative study of 102 eyes of 102 patients with chronic glaucoma or ocular hypertension. Intraocular pressure (IOP) measurements by the Goldmann tonometer (GT) were compared with self-measured readings with the PP...

To date, only bar/pat gene derived from Streptomyces has been used to generate the commercial PPT-resistant crops currently available in the market. The limited source of bar/pat gene is probably what has caused the decrease in PPT-tolerance, which has become the main concern of those involved in field management programs. Although glutamine synthetase (GS) is the target enzyme of PPT, little s...

Three hundred bacterial isolates from soil were tested for resistance against phosphinothricin [PPT; dl-homoalanin-4-yl(methyl)phosphinic acid], the active ingredient of the herbicide BASTA. Eight resistant bacterial strains and Escherichia coli were analyzed for PPT-transforming activities. At least three different enzymatic reactions could be detected in cell extracts. In six strains an acety...

Potato purple top (PPT) is an emerging potato disease complex. The etiological agents of PPT are phloem-inhabiting, cell wallless bacteria known as phytoplasmas. Tomato is an alternative host of these phytoplasmas. PPT phytoplasma-infected tomato plants exhibit symptoms including abnormal foliage development and formation of “big buds”. In the present study, twodimensional gel electrophoresis (...

Recently, two types of fatty acid synthases (FASs) have been discovered from apicomplexan parasites. Although significant progress has been made in characterizing these apicomplexan FASs, virtually nothing was previously known about the activation and regulation of these enzymes. In this study, we report the discovery and characterization of two distinct types of phosphopantetheinyl transferase...