Background: Proteolytic enzymes have an important role in variety of physiological and pathological functions. They have been used in therapeutic and pharmaceutical applications. Characterizations of extracellular proteases from various strains of S. marcescens indicate that most strains produce a very similar major metalloprotease. This metalloprotease (serrapeptidase, serrapeptase) is an impo...

The production of protease by Bacillus licheniformis BBRC 100053 was studied. The most appropriate medium for the growth and protease production is composed of: lactose 1%, yeast extract 0.5%, peptone 0.5%, KH2PO4 0.1%, MgSO4.7H2O 0.02%. Enzyme production corresponded with growth and reached a maximums level (589 U/ml) during the stationary phase at 35°C, pH equivalent to 10 and with 150 rpm af...

This study was conducted to investigate the effects of protease supplementation on behaviour, slaughter performance, meat quality, and immune organ indices broilers. A total 240 one-day-old Arbor Acres broiler chickens were randomly allocated four treatments, with six replicates per treatment 10 broilers replicate. Chickens fed corn-soybean meal basal diets supplemented 0, 4, 8, 12 IU/g p...

The present study explored the efficacy of Bacillus spp. and protease production for biocontrol of the root-knot nematode Meloidogyne javanica in African violet media. Among 100 bacterial isolates from various soils, the highest nematode mortality was observed for treatments with isolate GM-18, which was identified as Bacillus subtilis based on cultural and morphological characteristics and 16S...

Penelitian ini bertujuan mengeksplorasi senyawa kimia kulit kopi sebagai anti Covid-19 (anti-3C-like protease). Analisis in silico dilakukan dengan tahapan pengambilan struktur dan protein, preparasi penginteraksian protein 3CL-Pro. Docking program Molegro virtual Docker 5 divisualisasi Discovery Studio versi 21.1.1. Senyawa yang terkandung dalam kopi, yaitu quinic acid, gluconic acid maupun fe...

in this study, the effect of accelerated ripening with encapsulated protease and lipase on physiochemical properties such as fat and moisture, texture and microstructure of brined cheese was investigated. addition of lipase increases hardness by reducing fat globules diameter and increasing casein matrix junction. it also decreases brittleness by fat degradation which is responsible for plastic...

Using as a template the crystal structure of SARS-CoV-2 main protease, we developed pharmacophore model functional centers protease inhibitor-binding pocket. With this model, conducted data mining conformational database FDA-approved drugs. This search brought 64 compounds that can be potential inhibitors protease. The conformations these undergone 3D fingerprint similarity clusterization. Then...

the aim of this study was to clone the serine alkaline protease-encoding gene from bacillus subtilis 168. this protease, which can have many applications especially in detergent, may be industrially an important enzyme. for the amplification of the gene, pcr was performed with a pair of primers specifically designed for this purpose. electrophoresis of the pcr product showed the expected band o...