Modern methods of pest control have tended to rely on a single method, whether it be a pesticide or a gene coding for resistance embedded using modern biotechnology tools in the crop itself. An important advantage of integrated pest management (IPM) over reliance on a single tool is that the repeated application of the single method rapidly leads to pest resistance to the tool. Using a variety ...

background: the advent of recombinant dna technology has facilitated heterologous expression of proteins from various sources in different host systems including escherichia coli. if a plant virus coat protein is expressed in the bacterium it can be used as the antigen for antibody preparation. such a recombinant antigen preparation can be particularly useful where equipment such as ultracentri...

Pooled expertise focused on the plant – this interdisciplinary approach defines the Julius BTL Bio-Test Labor GmbH Sagerheide was founded in 1993 and is located in Sagerheide (Northern Germany near Rostock). The company has set its priorities on the development of methods and execution of examinations for the registration of pesticides and transgenic plants. BTL develops suitable methods for br...

Potato virus Y group C isolates (PVYC) have been characterized according to biological, molecular and genetic criteria. Two genetic strains, PVYC1 and PVYC2, were identified on the basis of genetic distances (among them and other PVY strains), host range (ability or inability to infect pepper), MAb response (ELISA recognition with MAb 10E3) and coat protein processing site. Some characteristics...

A reverse transcription polymerase chain reaction (RT-PCR) based protocol with a pair of potato virus Y coat protein gene specific primers was found to be more sensitive method for the PVY detection in infected tobacco leaf and potato tuber tissues than conventional enzyme linked immunosorbent assay (ELISA) or nucleic acid spot hybridization (NASH) based methods. It was found in our studies tha...