نتایج جستجو برای: rapd analysis

تعداد نتایج: 2827449  

Journal: :Veterinary microbiology 2001
N Chansiripornchai P Ramasoota J Sasipreeyajan S B Svenson

Here we describe the application of a random amplified polymorphic DNA (RAPD) analysis for molecular genetic typing avian pathogenic Escherichia coli (APEC) strains. The RAPD technique was shown to be highly reproducible. Stable banding patterns with a high discriminatory capacity were obtained using two different primers. Overall, 55 E. coli strains were analyzed with a RAPD technique. The RAP...

Journal: :Journal of microbiology and biotechnology 2010
Islam Nazrul Mahmudul Yin-Bing Bian

Morphologically, nine different slow-growing protoclones were screened from regenerated protoplast of heterokaryotic Agaricus bisporus. The present study is the first report of fingerprinting on differentiating homo- and heterokaryotic protoclones using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. Among 80 primers tested, the seven ISSR and seven RAPD...

2007
Yanqing Zhou Jianjun Li Fang Wang Fengping Gu Chune Zhou Zhongyi Zhang Zhiming Gao

Two types of molecular markers, random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR), were assayed to determine the genetic diversity of ten experimental materials in Rehmannia glutinosa Libosch .f. hueichingensis (Chao et Schih) Hsiao, including eight cultivars and two virus-free lines micropropagated by tip tissue culture from Wenxian county. A high level of polymor...

Journal: :Clinical molecular pathology 1995
C J Linton A D Smart J P Leeming H Jalal A Telenti T Bodmer M R Millar

Aim-To compare restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) methods for the epidemiological typing of Mycobacterium tuberculosis.Methods-Thirty one M tuberculosis cultures originating from patients in the Canton of Berne in Switzerland, which had previously been typed by RFLP, were subjected to RAPD analysis. Cultures were coded so that the investi...

2016
Sina Mobasherizadeh Hasan Shojaei Seyed Asghar Havaei Kamyar Mostafavizadeh Fazollah Davoodabadi Farzin Khorvash Behrooz Ataei Abbas Daei-Naser

The aim of this study was to apply RAPD technique to analyze the genetic variability among the Iranian CA-MRSA isolates.The RAPD amplification was implemented on 25 strains isolated from the anterior nares of 410 healthy children using four randomly selected oligonucleotide primers from the stocks available in our laboratory, including the primers 1254, GE6, OLP6 and OLP13 from our stock. The a...

Journal: :FEMS microbiology letters 2007
Mitsuo Ogura Yasutaro Fujita

The Bacillus subtilis genome encodes eleven Rap proteins, which are conserved tetratricopeptide-containing regulatory proteins. Of those characterized to date, all except RapI negatively regulate response regulators, including Spo0F, ComA and DegU, via protein-protein interactions. RapD has not yet been fully characterized. It was examined whether RapD inhibits the expression of spoIIE, srfA an...

Journal: :Journal of clinical microbiology 1993
B G Corney J Colley S P Djordjevic R Whittington G C Graham

We compared random amplified polymorphic DNA (RAPD) fingerprinting with cross-absorption agglutination and restriction enzyme analysis for typing bovine leptospires. Using RAPD fingerprinting, we examined a number of Leptospira serovars, namely, hardjo genotypes bovis and prajitno, pomona, balcanica, tarassovi, swajizak, kremastos, australis, and zanoni, which are likely to be isolated from Aus...

Journal: :Journal of applied microbiology 1997
M T Momol E A Momol W F Lamboy J L Norelli S V Beer H S Aldwinckle

The genetic diversity among 16 strains of Erwinia amylovora, chosen to represent different host plant origins and geographical regions, was investigated by RAPD analysis. One strain of Erwinia herbicola and one of Agrobacterium vitis were used as outgroups. Ninety-eight different RAPD fragments were produced by polymerase chain reaction amplification with six different 10-mer primers. RAPD band...

Journal: :Biological research 2007
Monique Mancuso Rubén Avendaño-Herrera R Zaccone Alicia E Toranzo Beatriz Magariños

This study evaluates the effectiveness of three different molecular techniques, repetitive extragenic palindromic PCR (REP-PCR), enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) and the random amplified polymorphic DNA (RAPD-PCR) for rapid typing of Photobacterium damselae ssp. piscicida strains isolated from different species of marine fish and geographic areas. The resu...

Journal: :Journal of clinical microbiology 1999
S Chatellier M Gottschalk R Higgins R Brousseau J Harel

The genetic diversity of 88 Streptococcus suis serotype 2 isolates which were recovered from various countries was examined by randomly amplified polymorphic DNA (RAPD) analysis with three primers. This bacterial collection included 80 isolates of porcine origin and 8 of human origin. This investigation allowed the identification of 23 RAPD types containing 1 to 30 isolates originating from one...

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