to detect the presence of infectious bronchitis virus (ibv) in infected allantoic fluid (af) of spf embryonated eggs rapid hemagglutination (ha) activity after treatment with neuraminidase enzyme was used. twenty ibv suspected materials were inoculated in spf embryonated eggs via chorioallantoic cavity. harvested afs were treated with neuraminidase enzyme and the presence of ibv was detected by...

To detect the presence of infectious bronchitis virus (IBV) in infected allantoic fluid (AF) of SPF embryonated eggs rapid hemagglutination (HA) activity after treatment with neuraminidase enzyme was used. Twenty IBV suspected materials were inoculated in SPF embryonated eggs via chorioallantoic cavity. Harvested AFs were treated with neuraminidase enzyme and the presence of IBV was detected by...

Ten Pasteurella haemolytica isolates from the nasal passages of cattle, untypable by the indirect hemagglutination procedure, were grouped into three serotypes by the rapid plate agglutination procedure. Serological specificity and serum absorption results showed that the 10 indirect hemagglutination-negative isolates belonged to three distinct serotypes and were not members of established sero...

Antibody titers to tetanus toxin in human sera were assayed by passive hemagglutination with turkey erythrocytes, enzyme-linked immunosorbent assay, and counterimmunoelectrophoresis. The first two of these tests were shown to be the most sensitive for antibody detection, having the same range of sensitivity and reproducibility. The antibody levels determined by these assays were up to 400-fold ...

Sera from 628 nonsyphilitic individuals were tested with the Rapid Plasma Reagin-Card, Fluorescent Treponemal Antibody-Absorbed, and Hemagglutination Treponemal Test for Syphilis tests to ascertain the comparative specificity of these tests. Many sera were also tested with the quantitative Venereal Disease Research Laboratory test. Sera included in the study were from both normal individuals an...

BACKGROUND AND PURPOSE Mycoplasma pneumoniae infection is a frequent cause of community-acquired respiratory infections in children and adults. However, standardized, rapid, specific methods for its diagnosis are lacking. The relationship between M. pneumoniae infection and asthma exacerbation has been recently discussed in the literature. We investigated the accuracy of rapid detection of myco...

A rapid, simple, and accurate counterimmunoelectrophoresis (CIE) technique was developed and compared with the indirect hemagglutination and rapid plate agglutination techniques for serotyping cultures of Pasteurella haemolytica. The CIE test had 100% correlation with the conventional indirect hemagglutination test and, after serum absorption, correctly identified cultures representing the 12 e...